ABSTRACT

The present investigation aims to explore therapeutic potential of biogenic synthesised silver nanoparticles (AgNPs) mediated using endophytic fungus extract isolated from Tinospora cordifolia. The synthesised AgNPs characterised using various physicochemical analyses. In FTIR, amide I and II bands of protein and hydroxyl groups of phenolic hydroxyl confirming bioactive metabolites presence over AgNPs surface. The mean size of AgNPs found 25–35 nm spherical in size. Further, AgNPs demonstrated higher antibacterial, antioxidant, and anti-inflammatory activity than endophyte fungal extract. Such high activity of AgNPs might be a resultant of the propounding synergistic activity of silver nanoparticle and endophyte bioactive metabolites over AgNPs surface that is confirmed by FTIR. Moreover, antiproliferative activity in cervical and breast cancer cells was demonstrated. Finally, AgNPs found cytocompatibility and hemocompatibility. Thus, the results obtained provide apparent and multiple biological activities of biogenic silver nanoparticles synthesised as possible multipurpose therapeutic material with little systemic toxicity.

摘要

本研究旨在探索使用从心叶黄孢菌中分离的内生真菌提取物介导的生物合成银纳米粒子 (AgNPs) 的治疗潜力. 使用各种物理化学分析表征合成的 AgNP。在 FTIR 中，蛋白质的酰胺 I 和 II 带以及酚羟基的羟基证实了 AgNPs 表面存在生物活性代谢物。AgNPs 的平均尺寸为 25-35 nm 球形。此外，AgNPs 表现出比内生真菌提取物更高的抗菌、抗氧化和抗炎活性。AgNPs 的如此高活性可能是银纳米颗粒和内生生物活性代谢物在 AgNPs 表面上的协同活性的结果，FTIR 证实了这一点。此外，证明了在宫颈癌细胞和乳腺癌细胞中的抗增殖活性。最后，AgNPs 发现了细胞相容性和血液相容性。因此，