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Morphological description, genetic diversity and population structure of safflower (Carthamus tinctorius L.) mini core collection using SRAP and SSR markers
Biotechnology & Biotechnological Equipment ( IF 1.4 ) Pub Date : 2020-01-01 , DOI: 10.1080/13102818.2020.1818620
Seyed Mohammad Reza Hassani 1 , Reza Talebi 1 , Sayyed Saeid Pourdad 2 , Amir Mohammad Naji 3 , Farzad Fayaz 1
Affiliation  

Abstract This study was conducted to investigate the genetic diversity and population structure of globally diverse mini core collection of safflower (Carthamus tinctorius L.) using agro-morphological, sequence-related amplified polymorphism (SRAP) and simple-sequence repeats (SSR) molecular markers. Field experiment results showed significant differences between genotypes for agro-morphological traits such as: number of capitula per plant, number of seeds per plant, 1000-seed weight and seed yield. Ten SRAP primer combinations generated 120 polymorphic bands with an average polymorphism information content (PIC) value of 0.23. High allelic variation using 10 SSR markers observed across genotypes, with an average value of 6.8 alleles per locus and relatively high value for PIC (0.69). Pooled molecular data (SSR + SRAP) were used to identify the population structure and genetic diversity using discriminant analysis of principal components (DAPC) and STRUCTURE analysis. The results revealed four clusters within the safflower accessions, while there was no strong correlation between geographical origins and estimated genetic diversity. Our results showed high genetic diversity for both agro-morphological and molecular analysis in the examined safflower germplasm. This mini core germplasm can be used for germplasm management and future safflower breeding programs.

中文翻译:

使用 SRAP 和 SSR 标记的红花 (Carthamus tinctorius L.) 迷你核心集合的形态学描述、遗传多样性和种群结构

摘要 本研究旨在利用农业形态学、序列相关扩增多态性 (SRAP) 和简单序列重复 (SSR) 分子标记研究全球多样化的红花微型核心集落的遗传多样性和种群结构。 . 田间试验结果表明,单株头状花序数、单株种子数、千粒重和种子产量等农业形态性状基因型之间存在显着差异。十个 SRAP 引物组合产生了 120 个多态性条带,平均多态性信息含量 (PIC) 值为 0.23。使用 10 个 SSR 标记在基因型间观察到的高等位基因变异,每个基因座的平均值为 6.8 个等位基因,PIC 的值相对较高 (0.69)。汇总分子数据(SSR + SRAP)用于使用主成分判别分析(DAPC)和结构分析来识别种群结构和遗传多样性。结果显示红花种质中有四个簇,而地理起源和估计的遗传多样性之间没有很强的相关性。我们的结果表明,在所检查的红花种质中,农业形态学和分子分析都具有很高的遗传多样性。这种微型核心种质可用于种质管理和未来的红花育种计划。虽然地理起源和估计的遗传多样性之间没有很强的相关性。我们的结果表明,在所检查的红花种质中,农业形态学和分子分析都具有很高的遗传多样性。这种微型核心种质可用于种质管理和未来的红花育种计划。虽然地理起源和估计的遗传多样性之间没有很强的相关性。我们的结果表明,在所检查的红花种质中,农业形态学和分子分析都具有很高的遗传多样性。这种微型核心种质可用于种质管理和未来的红花育种计划。
更新日期:2020-01-01
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