Abstract

In Asia, uneaten cooked rice is the highest portion amongst many forms of food wastes that are thrown away. In order to make use of the thrown-away rice and potentially use it for liquid fuels, steamed Japanese rice was evaluated on biobutanol production through a two-step fermentation by amylase-producing Aspergillus oryzae, and solvent-producing Clostridium acetobutylicum YM1. The effects of sterilization and providing anaerobic conditions on solvent production in acetone-butanol-ethanol (ABE) fermentation cannot be underestimated. Several conditions, including aerobic, anaerobic, sterile, and non-sterile were investigated concerning the solvent production capability of Clostridium acetobutylicum YM1. The maximum solvent production was 11.02 ± 0.22 g/l butanol and 18.03 ± 0.34 g/l total ABE from 75 g/l dried rice. The results confirmed that the solvent production performance of the YM1 strain was not affected by the sterilization conditions. In particular, 10.91 ± 0.16 g/l butanol and 16.68 ± 0.22 g/l ABE were produced under non-sterile and aerobic conditions, which can reduce industrial-scale production costs.

摘要

在亚洲，未食用的米饭是被丢弃的多种食物垃圾中最高的部分。为了利用被丢弃的大米并可能将其用作液体燃料，通过生产淀粉酶的米曲霉和生产溶剂的丙酮丁醇梭菌YM1 的两步发酵，对蒸过的日本大米进行了生物丁醇生产评估。灭菌和提供厌氧条件对丙酮-丁醇-乙醇 (ABE) 发酵中溶剂生产的影响不可低估。研究了需氧、厌氧、无菌和非无菌等几种条件下丙酮丁醇梭菌的溶剂生产能力。YM1。75 g/l 干米的最大溶剂产量为 11.02 ± 0.22 g/l 丁醇和 18.03 ± 0.34 g/l 总 ABE。结果证实，YM1菌株的溶剂生产性能不受灭菌条件的影响。特别是在非无菌和好氧条件下生产了10.91±0.16 g/l的丁醇和16.68±0.22 g/l的ABE，可以降低工业规模的生产成本。