Abstract

Aim

MUC-1-peptide (M-1-pep) loaded poly (lactide-co-glycolide) nanoparticles were coated with protamine sulphate (PS), M-1-pep-PS-P-NPs for targeting antigen presenting cells (APCs) to evoke cytokine release.

Methods and results

M-1-pep-PS-P-NPs were tailored by emulsion-diffusion evaporation method and characterised in vitro under a set of rigorous parameters. The average particle size and zeta potential of optimised M-1-pep-PS-P-B-NPs was measured to be 132.21 ± 30.71 nm and 6.29 ± 0.71 mV, significantly (p < 0.01) higher than 71.24 ± 17.76-nm and −43.41 ± 3.37 mV of M-1-pep-P-NPs. Further, 50-μg/ml concentration of M-1-pep-PS-P-B-NPs displayed 82.4% cellular uptake in RAW 264.7 cells calculated in setting of fluorescence intensity significantly (p < 0.05) elevated than 63.1% of M-1-pep-P-NPs. Consistent to quantitative results, M-1-pep-PS-P-B-NPs also confirmed advanced cellular uptake (CU) in RAW 264.7 cells in contrast to M-1-pep-P-NPs suppose to be through multiple mechanisms including phagocytosis and clathrin mediated endocytosis.

Conclusion

M-1-pep-PS-P-B-NPs must be evaluated in vivo through inhalation route of administration for antitumor prospective in lung cancer xenograft model.

中文翻译：

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MUC-1 肽的硫酸鱼精蛋白包被的聚（丙交酯-共-乙交酯）纳米颗粒改善了小鼠抗原呈递细胞中的细胞摄取和细胞因子释放。

摘要

目的

MUC-1-肽（M-1-pep）负载的聚（丙交酯-共-乙交酯）纳米粒子涂有硫酸鱼精蛋白（PS），M-1-pep-PS-P-NPs用于靶向抗原呈递细胞（APCs）引起细胞因子的释放。

方法和结果

M-1-pep-PS-P-NPs 是通过乳液扩散蒸发方法定制的，并在一组严格的参数下进行体外表征。优化后的 M-1-pep-PS-PB-NPs 的平均粒径和 zeta 电位测得为 132.21 ± 30.71 nm 和 6.29 ± 0.71 mV，显着 ( p  < 0.01) 高于 71.24 ± 17.76-nm 和 -43.41 ± 3.37 mV 的 M-1-pep-P-NP。此外，50-μg/ml 浓度的 M-1-pep-PS-PB-NPs 在 RAW 264.7 细胞中显示 82.4% 的细胞摄取，在荧光强度设置中显着（p < 0.05) 高于 63.1% 的 M-1-pep-P-NP。与定量结果一致，M-1-pep-PS-PB-NPs 还证实了 RAW 264.7 细胞中的高级细胞摄取 (CU)，而 M-1-pep-P-NPs 假设通过多种机制，包括吞噬作用和网格蛋白介导的内吞作用。

结论

M-1-pep-PS-PB-NPs 必须通过吸入给药途径在体内进行评估，以便在肺癌异种移植模型中进行抗肿瘤预期。