Influence of Solvents on Steroid Extraction from Animal Feces and Quantification Using Enzyme Immunoassay,Wildlife Society Bulletin

当前位置： X-MOL 学术 › Wildl. Soc. Bull. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Influence of Solvents on Steroid Extraction from Animal Feces and Quantification Using Enzyme Immunoassay
Wildlife Society Bulletin ( IF 0.9 ) Pub Date : 2020-09-15 , DOI: 10.1002/wsb.1124
Yihua Wang ₁ , Xiaolong Hu ₂ , Gang Liu ₃ , Defu Hu ₄ , Dongping Liu ₁

Affiliation

Organic solvents, such as methanol (MeOH) and ethanol (EtOH), are commonly used to extract and preserve steroids from animal excreta (feces, urine). As a result, the target substances are often in the solvent when they are subsequently analyzed using enzyme immunoassay (EIA). We examined steroid extraction from the feces of forest musk deer (Moschus berezovskii; collected in Sichuan Province, China, during 2017) using different concentrations of MeOH or EtOH (60%, 80%, 90%, or 100%). We also tested the effects of EtOH solvent on the accuracy of the EIA used to determine steroid concentration. Our results show that 100% MeOH and 90% EtOH resulted in the extraction of greater cortisol content from the feces of female forest musk deer compared with 90% MeOH and 100% EtOH (P < 0.05). Greater estradiol content was extracted with 100% and 90% EtOH compared with other EtOH concentrations or MeOH. The largest testosterone amount was extracted using 90% EtOH. In our evaluation of the effects of EtOH concentration on the accuracy of the EIA test, we found that inaccurate results appeared when the sample EtOH concentration was >7%. © 2020 The Wildlife Society.

更新日期：2020-09-15

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11