The prebiotic effect of the agave plant polysaccharides (agavin) was evaluated to improve enteritis caused by soybean meal (SBM) in juveniles of Totoaba macdonaldi (60.8 ± 2.3 g). Four isoproteic and isolipidic diets were formulated: an FM‐based formulation as reference (RD), a blend of FM and PBM (FM‐PBM; 1:2 as previously shown better performance than FM), the blend combined with 24% SBM (SBM‐B) and, lastly, the latter combination with 2% agavin (SBM‐B + A). After 44 days, the SBM‐B + A treatment resulted in a definite enteritis improvement, besides a significantly higher final growth (207.1 ± 3.8 vs. 195 ± 3.4) compared to the rest of the treatments. According to the microbiota intestine structure, it can be revealed that agavin resulted in enrichment of the Sinorhizobium genera, characterized by nitrogen fixation. However, at a species level, Clostridium botyricum was the more prominent in PBM‐based feeds, known as beneficial for the suppression of inflammatory response. In contrast, Enterococcus cecorum was identified in the gut of fish fed SBM‐based feeds, intensified in agavin‐supplemented feeds. Higher expression of hsp70 and aqp8 was registered in FM‐PBM. It is concluded that agavin provides a clear benefit when added to diets. The implications of FM substitution are discussed.

中文翻译：

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十足的Totoaba，Totoaba macdonaldi日粮中的益生元龙舌兰可缓解大豆粉诱发的肠炎：生长性能，肠道组织学和微生物群

评估了龙舌兰植物多糖（agavin）的益生元作用，以改善Totoaba macdonaldi幼鱼（60.8±2.3 g）中由豆粕（SBM）引起的肠炎。配制了四种同质和异脂饮食：基于FM的配方作为参考（RD），FM和PBM的混合物（FM-PBM；以前显示为1：2的性能优于FM），混合物和24％SBM的混合物（SBM-B），最后是2％龙舌兰（SBM-B + A）。44天后，SBM-B + 与其他疗法相比，一种疗法可明显改善肠炎，并且最终生长显着提高（207.1±3.8对195±3.4）。根据微生物群的肠道结构，可以发现龙舌兰引起了中华根瘤菌属的富集，其特征是固氮。但是，在物种水平上，肉毒梭菌在基于PBM的饲料中更为突出，已知对抑制炎症反应有益。相比之下，在以SBM为基础的饲料喂养的鱼的肠中发现了肠球菌，而在以补充剂补充的饲料中则有所增强。在FM‐PBM中注册了hsp70和aqp8的高表达。结论是，在饮食中添加龙舌兰可以带来明显的好处。讨论了FM替代的含义。