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Placenta expression of vitamin D and related genes in pregnant women with gestational diabetes mellitus.
The Journal of Steroid Biochemistry and Molecular Biology ( IF 2.7 ) Pub Date : 2020-09-15 , DOI: 10.1016/j.jsbmb.2020.105754
Yanyan Wang 1 , Tian Wang 1 , Yan Huo 1 , Li Liu 1 , Suxin Liu 1 , Xiaoqian Yin 1 , Runfang Wang 1 , Xingshuang Gao 1
Affiliation  

Objectives and Methods

A total of 41 GDM and 40 normal glucose tolerance subjects were recruited. Through detecting the level of Serum vitamin D with electrochemical luminescence and vitamin D receptor (VDR) with Enzyme-linked immunosorbent assay (ELISA) in maternal and cord blood, the expression leves of CYP24A1, CYP27B1, VDR protein and mRNA in placenta and umbilical cord with western blotting and RT-PCR, and the DNA methylation levels of CYP24A1 and CYP27B1 gene in placenta with methylation-specific PCR (MSP) and direct bisulfite sequencing (BSP) analysis to explore the potential role of the vitamin D and its related genes in gestational diabetes mellitus (GDM).

Results

Serum vitamin D concentrations were significantly higher in normal pregnant than women with GDM in maternal blood (P < 0.01) and cord blood (P = 0.014). Compared to the control group, the expression levels of CYP24A1 protein (P < 0.01) and mRNA (P = 0.021) and VDR protein (P = 0.026) and mRNA (P = 0.023) in the GDM group were significantly higher in placenta and umbilical cord tissues (P = 0.015, P < 0.01, P = 0.028, P < 0.01, respectively), while that of CYP27B1 protein (P < 0.01) and mRNA (P = 0.042) was significantly lower (P = 0.022, P = 0.032, respectively). Moreover, partial DNA methylation of CYP24A1 and CYP27B1 genes was observed in both GDM and control groups.

Conclusions

Vitamin D deficiency participates in the pathogenesis of GDM, and changes in the expression of genes related to the vitamin D metabolic pathway are closely related to vitamin D levels in the pregnancy and fetus. However, DNA methylation of CYP24A1 and CYP27B1 might not be involved in the pathogenesis of GDM.



中文翻译:

妊娠糖尿病孕妇的胎盘中维生素D和相关基因的表达

目的与方法

总共招募了41名GDM和40名正常葡萄糖耐量受试者。通过与电化学发光和维生素d受体(检测血清维生素d的电平VDR)与母体和脐带血酶联免疫吸附测定(ELISA),表达leves CYP24A1CYP27B1VDR蛋白和mRNA在胎盘和脐带蛋白质印迹法和RT-PCR法检测胎盘中CYP24A1CYP27B1基因的甲基化水平,采用甲基化特异性PCR(MSP)和亚硫酸氢盐直接测序(BSP)分析,探讨维生素D及其相关基因在胎盘中的潜在作用妊娠糖尿病(GDM)。

结果

正常孕妇的血清中维生素D浓度明显高于GDM妇女的母血(P <0.01)和脐带血(P = 0.014)。与对照组相比,GDMCYP24A1蛋白(P <0.01)和mRNA(P = 0.021),VDR蛋白(P = 0.026)和mRNA(P = 0.022)的表达水平在胎盘和脐带明显升高。脐带组织(分别为P = 0.015,P <0.01,P = 0.028,P <0.01),而CYP27B1蛋白(P <0.01)和mRNA(P = 0.042)则明显降低(P = 0.022,P = 0.032) , 分别)。此外,在GDM组和对照组中均观察到CYP24A1CYP27B1基因的部分DNA甲基化。

结论

维生素D缺乏症参与GDM的发病机制,并且与维生素D代谢途径相关的基因表达的变化与孕妇和胎儿中的维生素D水平密切相关。然而,CYP24A1CYP27B1的DNA甲基化可能不参与GDM的发病机制。

更新日期:2020-09-22
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