Application of biochar has been widely suggested as a remediation tool for trace element-polluted soils, but the impact of biochar on microbial communities and on native plants remain largely unknown. To overcome this knowledge gap, biochar produced from rice husk and olive pit were applied at a rate of 8 t ha⁻¹ into a soil with two contrasting levels of trace elements (high and moderate) to study their effects on soil microbial community composition, vegetation cover and soil properties after 1, 6, 12 and 20 months under field conditions. Differences in bacterial community composition were studied using the Illumina Miseq technology of the 16S rRNA gene. Although variations in soil properties and ecological function were seasonal and soil-type dependent, biochar application enhanced soil properties and vegetation cover in the moderately polluted soil (MPS), and increased microbial diversity as well as vegetation cover in the highly polluted soil (HPS). Enzymatic activities and soil respiration rates were not modified with the application of biochar, but increased total carbon content of soils. The application of biochar from crop residues to trace-element contaminated soils provided environmental benefits, including plant diversity and growth, as well as the increase of bacterial diversity and carbon sequestration.

广泛建议使用生物炭作为修复微量元素污染土壤的工具，但是生物炭对微生物群落和天然植物的影响仍然未知。为了克服这一知识鸿沟，以稻壳和橄榄核生产的生物炭的施用量为8 t ha ^-1在两种不同水平的微量元素（高和中度）的土壤中，研究它们在田间条件下1、6、12和20个月后对土壤微生物群落组成，植被覆盖和土壤特性的影响。使用16S rRNA基因的Illumina Miseq技术研究了细菌群落组成的差异。尽管土壤性质和生态功能的变化取决于季节和土壤类型，但生物炭的施用可提高中度污染土壤（MPS）的土壤特性和植被覆盖率，并增加高污染土壤（HPS）的微生物多样性和植被覆盖率。酶活性和土壤呼吸速率未因使用生物炭而改变，但增加了土壤的总碳含量。