Ozone (O₃) is a short-lived molecule which can be produced in a controlled reaction when oxygen is exposed to electric discharge. In the last few decades, many publications dealing both with animals and humans reported beneficial effects of ozone administration linked to its immunomodulatory and protective role against cellular damage. This is the first work which brings insight into how ozone influences cells of neural lineage in vitro and hypothesizes the potential molecular and novel electromagnetic mechanisms behind its action. By using neural stem cells, we show that ozone, especially in concentrations of around 11 μg/mL, significantly increases the speed of neural cell migration. With much lower effects, it also increases cell proliferation and cytokine production. Results of this study, at least partly, explain the observed beneficial effects of ozone in diseases of the nervous system tested on animal models and in human clinical trials. Therefore, here described effects of ozone on cellular level represent a firm basis for further investigation of possible applications of ozone in regeneration of the nervous system.

臭氧（O ₃）是一种短寿命的分子，当氧气暴露于放电时，可以通过受控反应生成。在过去的几十年中，许多涉及动物和人类的出版物都报告了臭氧施用的有益作用，这与臭氧对细胞损伤的免疫调节作用和保护作用有关。这是第一个使人了解臭氧如何在体外影响神经谱系细胞的工作并假设了其作用背后的潜在分子和新型电磁机制。通过使用神经干细胞，我们发现臭氧，尤其是浓度约为11μg/ mL的臭氧，显着提高了神经细胞迁移的速度。效果要低得多，它还可以增加细胞增殖和细胞因子的产生。这项研究的结果至少部分解释了臭氧对动物模型和人体临床试验中所测神经系统疾病的有益作用。因此，这里描述的臭氧对细胞水平的影响为进一步研究臭氧在神经系统再生中的可能应用提供了坚实的基础。