The presence of excessive reactive oxygen species (ROS) after injuries to the enthesis could lead to cellular oxidative damage, high inflammatory response, chronic inflammation, and limited fibrochondral inductivity, making tissue repair and functional recovery difficult. Here, a multifunctional silk fibroin nanofiber modified with polydopamine and kartogenin was designed and fabricated to not only effectively reduce inflammation by scavenging ROS in the early stage of the enthesis healing but also enhance fibrocartilage formation with fibrochondrogenic induction in the later stages. The in vitro results confirmed the antioxidant capability and the fibrochondral inductivity of the functionalized nanofibers. In vivo studies showed that the multifunctional nanofiber can significantly improve the integration of tendon-bone and accelerate the regeneration of interface tissue, resulting in an excellent biomechanical property. Thus, the incorporation of antioxidant and bio-active molecules into extracellular matrix-like biomaterials in interface tissue engineering provides an integrative approach that facilitates damaged tissue regeneration and functional recovery, thereby improving the clinical outcome of the engineered tissue.

受伤后，存在过多的活性氧（ROS）可能导致细胞氧化损伤，高炎症反应，慢性炎症和有限的纤维软骨诱导性，从而使组织修复和功能恢复困难。在这里，设计和制造了一种用聚多巴胺和卡托菌素修饰的多功能丝素蛋白纳米纤维，不仅可以在修复早期阶段通过清除ROS来有效减少炎症，而且在后期可以通过纤维软骨生成诱导增强纤维软骨的形成。该体外结果证实了抗氧化能力和功能化纳米纤维的fibrochondral电感。体内研究表明，多功能纳米纤维可以显着改善肌腱-骨的整合并加速界面组织的再生，从而具有出色的生物力学性能。因此，在界面组织工程中将抗氧化剂和生物活性分子掺入细胞外基质样生物材料中提供了一种整合的方法，该方法有助于受损组织的再生和功能恢复，从而改善了工程组织的临床结果。