Antibodies against human platelets cause a variety of thrombocytopenic disorders, which lead to potentially fatal haemorrhage. Therefore, their prompt detection is mandatory for successful patient treatment. Solid phase red cell adherence (SPRCA) assay allows for platelet antibody detection widely. However, preparation of fresh platelets with HLA-I and human platelet antigens (HPA)1–5,15 genotyped as target cells is inconvenient and fresh platelets have a short shelf life. In this study, the lyophilised human platelets for antibody detection in SPRCA were prepared. Firstly, platelets were resuspended in lyophilisation buffer and freeze-dried. Then the characteristics of lyophilised platelet were analysed. Rehydrated platelets were recovered with a mean rate of 80.91% ± 2.87%, and still retained spherical morphology. Indirect flow cytometry showed that glycoproteins IIb/IIIa, Ia/IIa, Ib/IX, IV, CD109, and HLA class I were present on the surface of the lyophilised platelets at a comparable level to that of fresh platelets. The consistent results obtained with WHO reference reagents containing anti-HPA-1a, anti-HPA-3a, and anti-HPA-5b, as well as clinical samples from the same donors containing anti-HLA antibodies when reacting with lyophilised versus fresh platelets confirmed good antigenicity preservation of platelets after freeze-drying. Further investigation showed that the lyophilised platelets could be stored at 2–8 °C for up to 14 months and the reconstituted suspension was stable for 48 h. Therefore, lyophilised platelets can be a convenient alternative to fresh platelets to use for anti-platelet antibody detection in SPRCA tests.

针对人类血小板的抗体会导致多种血小板减少性疾病，从而导致潜在的致命性出血。因此，对于成功的患者治疗，必须迅速检测它们。固相红细胞粘附（SPRCA）分析可广泛检测血小板抗体。但是，用基因型作为靶细胞的HLA-1和人血小板抗原（HPA）1-5,15制备新鲜血小板是不方便的，并且新鲜血小板的保质期很短。在这项研究中，制备了用于SPRCA中抗体检测的冻干人血小板。首先，将血小板重悬于冻干缓冲液中并冷冻干燥。然后分析冻干血小板的特征。回收的复水血小板的平均率为80.91％±2.87％，并且仍保留球形形态。间接流式细胞仪显示糖蛋白IIb / IIIa，Ia / IIa，Ib / IX，IV，CD109和HLA I类以与新鲜血小板相当的水平存在于冻干血小板表面。当与冻干或新鲜血小板反应时，使用含有抗HPA-1a，抗HPA-3a和抗HPA-5b的WHO参考试剂以及含有抗HLA抗体的相同供体的临床样品获得了一致的结果冷冻干燥后对血小板的抗原性保持良好。进一步的研究表明，冻干的血小板可以在2–8°C下保存长达14个月，重新配制的悬浮液可以稳定放置48小时。因此，冻干血小板可以作为新鲜血小板的方便替代品，用于SPRCA测试中的抗血小板抗体检测。