Isolation of complex dissolved organic matter (DOM) from environmental water is a major challenge for unknown screening analysis by high-resolution mass spectrometry. In this study, DOM in process water during advanced drinking water treatment was fractionated sequentially by three solid-phase extraction (SPE) cartridges based on the polarity and charge of DOM molecules. By sequential SPE with unknown screening analysis, over 3000 DOM features were found in raw water, whereas around 2000 were obtained by a single SPE. The hydrophobic neutral (HPON) fraction contained CHO features with highest averaged molecular weight followed by hydrophobic acid (HPOA) and then hydrophilic acid (HPIA). The average degree of carbon double bond equivalents and carbon oxidation states indicated that the HPON fraction contained molecules that were more unsaturated and less oxidized than those of the HPOA and HPIA fractions. Ozone selectively decomposed (1) more unsaturated and less oxidized HPON features, (2) more unsaturated HPOA compounds, and (3) less oxidized HPIA molecules. Oxidation by-products were mostly HPON and HPIA compounds that were more oxidized than the decomposed molecules. During biological activated carbon (BAC) filtration, less oxidized HPON were preferentially removed, whereas HPOA were removed without selectivity. HPON and HPIA molecules with more oxidized character were found to be refractory to BAC treatment. HPON with more unsaturated and HPIA with more oxidized characters were decomposed by chlorine. Many types of HPIA decomposed during chlorination were the oxidation by-products of ozonation that were refractory to BAC treatment. Sequential SPE with unknown screening analysis provided previously unknown details of the molecular characteristics of DOM and its changes during advanced water treatment.

从环境水中分离复杂的溶解有机物（ DOM ）是高分辨率质谱法进行未知筛选分析的主要挑战。在这项研究中，通过三个固相萃取（ SPE ）顺序分离了高级饮用水处理过程中的过程水中的DOM。弹药基于DOM分子的极性和电荷。通过具有未知筛选分析的连续SPE，在原水中发现了3000多个DOM特征，而单个SPE则获得了约2000个DOM特征。疏水性中性（HPON）馏分包含平均分子量最高的CHO特征，其次是疏水性酸（HPOA），然后是亲水性酸（HPIA）。碳双键当量和碳氧化态的平均程度表明，HPON馏分包含的分子比HPOA和HPIA馏分的分子更不饱和且氧化程度更低。臭氧选择性分解（1）更多不饱和且氧化程度较低的HPON特征，（2）更多不饱和HPOA化合物，以及（3）氧化程度较低的HPIA分子。氧化副产物主要是HPON和HPIA化合物，它们比分解的分子氧化得更多。在生物活性炭（BAC）过滤过程中，优先去除氧化程度较低的HPON，而没有选择性地去除HPOA。发现具有更高氧化特性的HPON和HPIA分子对于BAC处理是难治的。具有更多不饱和键的HPON和具有更多氧化特征的HPIA被氯分解。在氯化过程中分解的许多类型的HPIA是臭氧氧化的副产物，对BAC处理是不利的。具有未知筛选分析的顺序SPE提供了先前未知的DOM分子特征及其在深度水处理过程中的变化的详细信息。优先去除氧化程度较低的HPON，而没有选择性地去除HPOA。发现具有更高氧化特性的HPON和HPIA分子对于BAC处理是难治的。具有更多不饱和键的HPON和具有更多氧化特征的HPIA被氯分解。在氯化过程中分解的许多类型的HPIA是臭氧氧化的副产物，对BAC处理是不利的。具有未知筛选分析的顺序SPE提供了先前未知的DOM分子特征及其在深度水处理过程中的变化的详细信息。优先去除氧化程度较低的HPON，而没有选择性地去除HPOA。发现具有更高氧化特性的HPON和HPIA分子对于BAC处理是难治的。具有更多不饱和键的HPON和具有更多氧化特征的HPIA被氯分解。氯化过程中分解的许多类型的HPIA是臭氧氧化的副产物，对BAC处理不利。具有未知筛选分析的顺序SPE提供了先前未知的DOM分子特征及其在深度水处理过程中的变化的详细信息。在氯化过程中分解的许多类型的HPIA是臭氧氧化的副产物，对BAC处理是不利的。具有未知筛选分析的顺序SPE提供了DOM分子特征及其在高级水处理过程中的变化的先前未知的细节。氯化过程中分解的许多类型的HPIA是臭氧氧化的副产物，对BAC处理不利。具有未知筛选分析的顺序SPE提供了DOM分子特征及其在高级水处理过程中的变化的先前未知的细节。