The novel natural low-molecular-mass polysaccharide (SLWPP-3) from pumpkin (Cucurbia moschata) was separated from the waste supernatant after macromolecular polysaccharide production and purified using a DEAE cellulose-52 column and gel-filtration chromatography. Chemical and instrumental studies revealed that SLWPP-3 with a molecular mass of 3.5 kDa was composed of rhamnose, glucose, arabinose, galactose and uronic acid with a weight ratio of 1: 1: 4: 6: 15, and primarily contained →3,6)-β-d-Galp-(1→, →4)-α-GalpA-(1→(OMe), →4)-α-GalpA-(1→, →2,4)-α-d-Rhap-(1→, →3)-β-d-Galp-(1→, →4)-α-d-Glcp, and →4)-β-d-Galp residues in the backbone. The branch chain passes were connected to the main chain through the O-4 atom of glucose and O-3 atom of arabinose. Physiologically, the ability of SLWPP-3 to inhibit carbohydrate-digesting enzymes and DPPH and ABTS radicals, as well as protect pancreatic β cells from oxidative damage by decreasing MDA levels and increasing SOD activities, was confirmed. The findings elucidated the structural types of pumpkin polysaccharides and revealed a potential adjuvant natural product with hypoglycemic effects.

在生产大分子多糖后，从废上清液中分离了来自南瓜（Cucurbia moschata）的新型天然低分子质量多糖（SLWPP-3），并使用DEAE纤维素52柱和凝胶过滤色谱法进行了纯化。化学和仪器研究表明，分子量为3.5 kDa的SLWPP-3由鼠李糖，葡萄糖，阿拉伯糖，半乳糖和糖醛酸组成，重量比为1：1：4：6：15，主要含有→3， 6）-β- d -Gal对-（1→，→4）-α-Gal的p A-（1→（OME），→4）-α-Gal的p A-（1→，→2,4） -α- d -RHA对-（1→，→3）-β- d -Gal对-（1→，→4）-α- d-Glc p和→4）-β- d- Gal p残基在主链中。支链通道通过葡萄糖的O-4原子和阿拉伯糖的O-3原子连接至主链。在生理上，证实了SLWPP-3抑制碳水化合物消化酶和DP​​PH和ABTS自由基，以及通过降低MDA水平和增加SOD活性来保护胰腺β细胞免受氧化损伤的能力。该发现阐明了南瓜多糖的结构类型，并揭示了具有降血糖作用的潜在佐剂天然产物。