Copper detection for diagnostic purposes is an appealing field due to the important biological role copper plays as a trace metal. A convenient strategy for sensing copper is to utilize its catalytic ability. Therefore, this review summarizes approaches for copper determination by Cu^I-catalyzed azide/alkyne cycloaddition (CuAAC). The concept was introduced in 2006 and all contributions made up to the middle of 2020 are covered in this review. The issue is divided into three categories: electrochemical, visual, and fluorescence-based methods. The advantages, as well as the disadvantages, of every group, are discussed in detail. The methodology which allows for the determination of copper content in water and human biological samples from 5 s up to 48 h without complex instrumentation are discussed. The reported range of limit of detection (LOD) was 0.38 aM–20 μM, with 1–10 nM being the typical range. The most successful strategies involved using DNA chains or enzymes in the sensing systems.

由于铜作为痕量金属起着重要的生物学作用，因此用于诊断目的的铜检测是一个吸引人的领域。感应铜的一种方便策略是利用其催化能力。因此，本综述总结了Cu ^I测定铜的方法-催化的叠氮化物/炔烃环加成反应（CuAAC）。该概念于2006年引入，此次审查涵盖了截至2020年中期的所有贡献。该问题分为三类：电化学方法，视觉方法和基于荧光的方法。详细讨论了每个组的优点和缺点。讨论了无需复杂仪器即可在5 s至48 h内测定水和人体生物样品中铜含量的方法。报告的检测限范围（LOD）为0.38 aM–20μM，典型范围为1–10 nM。最成功的策略涉及在传感系统中使用DNA链或酶。