Point-of-care (POC) diagnostic devices play significant roles in delivering vital surveillance information and providing proper and timely care to patients. There is a challenge in the development of new diagnostic tools to overcome their current shortcomings in terms of cost issues, accuracy and performance. Herein, a highly efficient paper-based analytical device based on a 2D metal-organic framework (MOF) has been reported for the colorimetric/fluorometric monitoring of glucose. Because of the inherent bifunctional activity of cobalt-terephthalate MOF (CoMOF) nanosheets, great improvements were made to the stability and performance of glucose oxidase (GOX) and to its catalytic effect on the reaction of o-phenylenediamine (OPD) and H2O2. The exceptional behavior of 2D CoMOF, along with a precise smartphone readout, led to the rapid and sensitive colorimetric/fluorometric detection of glucose in biological samples. Paper modified by CoMOF and GOX was stable for a long time, and a yellow-brown color and a high fluorescence emission were observed after the addition of a low volume of sample and OPD solutions. The probe showed a wide linear effectiveness range of 50 μM-15 mM, with colorimetric and fluorometric detection limits of 16.3 and 3.2 μM, respectively. Despite its great simplicity, the developed probe showed high performance and accuracy for the quantification of glucose.

中文翻译：

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嵌入纸上的双功能二维钴金属有机框架作为即时诊断设备：用于葡萄糖定量的应用

即时 (POC) 诊断设备在提供重要的监测信息和为患者提供适当和及时的护理方面发挥着重要作用。在开发新的诊断工具以克服其当前在成本问题、准确性和性能方面的缺点方面存在挑战。在此，已经报道了一种基于二维金属有机框架 (MOF) 的高效纸基分析装置，用于葡萄糖的比色/荧光监测。由于对苯二甲酸钴MOF（CoMOF）纳米片固有的双功能活性，葡萄糖氧化酶（GOX）的稳定性和性能及其对邻苯二胺（OPD）和H2O2反应的催化作用得到了极大的改善。2D CoMOF 的特殊行为，以及精确的智能手机读数，导致生物样品中葡萄糖的快速和灵敏的比色/荧光检测。CoMOF和GOX改性的纸长期稳定，在加入少量样品和OPD溶液后观察到黄棕色和高荧光发射。该探针显示出 50 μM-15 mM 的广泛线性有效性范围，比色和荧光检测限分别为 16.3 和 3.2 μM。尽管非常简单，但开发的探针在葡萄糖定量方面表现出高性能和准确性。该探针显示出 50 μM-15 mM 的广泛线性有效性范围，比色和荧光检测限分别为 16.3 和 3.2 μM。尽管非常简单，但开发的探针在葡萄糖定量方面表现出高性能和准确性。该探针显示出 50 μM-15 mM 的宽线性有效性范围，比色和荧光检测限分别为 16.3 和 3.2 μM。尽管非常简单，但开发的探针在葡萄糖定量方面表现出高性能和准确性。