Eighteen physio-biochemical traits influencing seed vigour were studied for their association with molecular markers using a mini core set constituted from 120 germplasm lines. High genetic variation was detected in the parameters namely chlrophyll a, Chlrophyll b, total chlorophyll, carotenoids, total anthocyanin content, gamma-oryzanols, total phenolics content, superoxide dismutase, catalase, guaicol peroxidase, total soluble sugar, total protein, seed vigour index -I and seed vigour index -II. Strong positive correlation of seed vigour index II was observed with amylose content, total anthocyanin content, catalase, total phenolic content and total flavonoid content while a negative association was observed for gamma-oryzanol content. High gene diversity (0.7169) and informative markers value (0.6789) were estimated from the investigation. Three genetic structure groups were observed in the panel population and genotypes were grouped in the subpopulations based on the seed vigour trait. Differences in the fixation indices of the three sub populations indicated existence of linkage disequilibrium in the studied panel population. Association of the traits namely total flavonoids, superoxide dismutase, catalase, chlorophyll a, Chlorophyll b, total chlorophyll, carotenoids, starch, amylose, total anthocyanin, gamma-oryzanol, total phenolics with the molecular markers were detected by Generalized Linear Model and Mixed Linear Model showing > 0.10 R² value. Association of the trait, total flavonoids with marker RM7364 located on chromosome 8 reported in earlier study was validated in this investigation. The validated markers and the novel markers detected showing higher R² value will be useful for improvement of seed vigour in rice.

使用由 120 个种质系组成的迷你核心组，研究了影响种子活力的 18 个生理生化性状与分子标记的关联。叶绿素a、叶绿素b、总叶绿素、类胡萝卜素、总花青素含量、γ-谷维素、总酚含量、超氧化物歧化酶、过氧化氢酶、愈创木酚过氧化物酶、总可溶性糖、总蛋白、种子活力指数等参数检测到高度遗传变异。 -I和种子活力指数-II。种子活力指数II与直链淀粉含量、总花青素含量、过氧化氢酶、总酚含量和总黄酮含量呈强正相关，与γ-谷维素含量呈负相关。从调查中估计出高基因多样性 (0.7169) 和信息标记值 (0.6789)。在小组群体中观察到三个遗传结构组，并根据种子活力性状对亚群体中的基因型进行分组。三个亚群体的固定指数的差异表明所研究的群体中存在连锁不平衡。采用广义线性模型和混合线性模型检测总黄酮、超氧化物歧化酶、过氧化氢酶、叶绿素a、叶绿素b、总叶绿素、类胡萝卜素、淀粉、直链淀粉、总花青素、γ-谷维素、总酚等性状与分子标记的关联性模型显示 > 0.10 R ²值。本次调查验证了早期研究中报道的总黄酮性状与位于 8 号染色体上的标记 RM7364 之间的关联。经验证的标记和检测到的显示较高R ²值的新型标记将有助于提高水稻种子活力。