A selectively modified porous metal/carbon nanocomposite was fabricated to enhance the enrichment of low-abundance phosphopeptides from biological samples. The carbon matrix derived from the metal-organic framework provides a suitable pore size to allow the diffusion of peptides, while the deliberately modified metal nanoparticles within the pores enhance their interaction with the phosphopeptides. This nanocomposite shows extremely high enrichment selectivity for phosphopeptides in the MALDI-TOF MS detection, even when the molar ratio of α-casein digests versus bovine serum albumin digests was up to about 1:20,000. By combining such nanocomposite with nano-LC-MS/MS, 4556 unique phosphopeptides were identified with high selectivity (95.2%) from HeLa cell extracts. Furthermore, phosphopeptides from prostate tissue digests were also determined. A total of 277 and 1242 phosphopeptides were identified from normal and tumor tissues of a patient with prostate cancer, respectively. This indicates that phosphorylation and prostate cancer can be related to each other.

中文翻译：

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钛 (IV) 靶向固定在源自金属-有机框架的磁性介孔纳米材料上，用于生物样品中的高效磷酸肽富集

制造了一种选择性改性的多孔金属/碳纳米复合材料，以增强生物样品中低丰度磷酸肽的富集。源自金属有机骨架的碳基质提供了合适的孔径以允许肽的扩散，而孔内有意修饰的金属纳米颗粒增强了它们与磷酸肽的相互作用。这种纳米复合材料在 MALDI-TOF MS 检测中对磷酸肽显示出极高的富集选择性，即使 α-酪蛋白消化物与牛血清白蛋白消化物的摩尔比高达约 1:20,000。通过将这种纳米复合材料与纳米 LC-MS/MS 相结合，从 HeLa 细胞提取物中以高选择性 (95.2%) 鉴定了 4556 种独特的磷酸肽。此外，还测定了来自前列腺组织消化物的磷酸肽。从一名前列腺癌患者的正常组织和肿瘤组织中分别鉴定出 277 和 1242 种磷酸肽。这表明磷酸化和前列腺癌可能相互关联。