“Halomonas socia” NY-011, a new species of moderately halophilic bacteria isolated and identified in our laboratory, can grow in high concentrations of salt ranging from 0.5 to 25%. In this study, the whole genome of NY-011 was sequenced and a detailed analysis of the genomic features was provided. Especially, a series of genes related to salt tolerance and involved in xenobiotics biodegradation were annotated by COG, GO and KEGG analyses. Subsequently, RNA-Seq-based transcriptome analysis was applied to explore the osmotic regulation of NY-011 subjected to high salt stress for different times (0 h, 1 h, 3 h, 6 h, 11 h, 15 h). And we found that the genes related to osmoregulation including excluding Na⁺ and accumulating K⁺ as well as the synthesis of compatible solutes (alanine, glutamate, ectoine, hydroxyectoine and glycine betaine) were up-regulated, while the genes involved in the degradation of organic compounds were basically down-regulated during the whole process. Specifically, the expression trend of genes related to osmoregulation increased firstly then dropped, which was almost opposite to that of degrading organic pollutants genes. With the prolongation of osmotic up-shock, NY-011 survived and gradually adapted to osmotic stress, the above-mentioned two classes of genes slowly returned to normal expression level. Then, the scanning electron microscope (SEM) and transmission electron microscope (TEM) were also utilized to observe morphological properties of NY-011 under hypersaline stress, and our findings showed that the cell length of NY-011 became longer under osmotic stress, at the same time, polyhydroxyalkanoates (PHAs) were synthesized in the cells. Besides, physiological experiments confirmed that NY-011 could degrade organic compounds in a high salt environment. These data not only provide valuable insights into the mechanism of osmotic regulation of NY-011; but also make it possible for NY-011 to be exploited for biotechnological applications such as degrading organic pollutants in a hypersaline environment.

“盐单胞菌socia ” NY-011，分离并在我们的实验室确定的中度嗜盐菌的一个新物种，可以在高浓度的盐从0.5％到25％的增长。在这项研究中，对NY-011的整个基因组进行了测序，并提供了对基因组特征的详细分析。尤其是，COG，GO和KEGG分析注释了一系列与耐盐性有关的基因，并参与了异种生物的生物降解。随后，基于RNA-Seq的转录组分析被用于探索在高盐胁迫下不同时间（0小时，1小时，3小时，6小时，11小时，15小时）的NY-011的渗透调节。我们发现与渗透调节有关的基因包括排除Na ⁺和积累K ⁺以及相容性溶质的合成（丙氨酸，谷氨酸，ectoine，羟基ectoine和甘氨酸甜菜碱）被上调，而参与有机化合物降解的基因在整个过程中基本被下调。具体而言，渗透压调节相关基因的表达趋势先升高后下降，这与降解有机污染物基因的趋势几乎相反。随着渗透休克的延长，NY-011存活下来并逐渐适应了渗透胁迫，上述两类基因逐渐恢复到正常表达水平。然后，还利用扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察高盐胁迫下NY-011的形貌，我们的研究结果表明，在渗透胁迫下，NY-011的细胞长度变长，同时，细胞中合成了聚羟基链烷酸酯（PHAs）。此外，生理实验证实，NY-011可以在高盐环境中降解有机化合物。这些数据不仅为了解NY-011的渗透调节机制提供了有价值的见解；同时也使NY-011可以用于生物技术应用，例如在高盐环境中降解有机污染物。