In the present work, bioethanol was produced by sugar fermentation obtained from water hyacinth using a novelty hybrid method composed of steam explosion and enzymatic hydrolysis, using hydrolytic enzymes produced by solid-state fermentation and water hyacinth as substrate. The highest activity, 42 U for xylanase and 2 U for cellulase per gram of dry matter, respectively, was obtained. Steam explosion pretreatment was performed at 190 ℃ for 1, 5, and 10 min, using water hyacinth sampled from the Maria Lizamba Lagoon, the Arroyo Hondo and the Amapa River. The highest amounts of reducing sugars of water hyacinth were obtained form the samples from the lagoon (5.4 g/50 g of dry matter) after 10 min of treatment. Steamed biomass was hydrolysed using the enzymes obtained by solid-state fermentation, obtained reducing sugars (maximum 15.5 g/L); the efficiency of enzymatic hydrolysis was 0.51 g of reducing sugars per gram of water hyacinth. Finally, reducing sugars were fermented using Saccharomyces cerevisiae for conversion to ethanol, with the highest ethanol concentration (7.13 g/L) and an ethanol yield of 0.23 g/g of dry matter.

在目前的工作中，利用固态发酵产生的水解酶和水葫芦作为底物，使用由蒸汽爆破和酶水解组成的新型混合方法，通过从水葫芦中获得的糖发酵生产生物乙醇。获得最高活性，分别为每克干物质 42 U 木聚糖酶和 2 U 纤维素酶。使用从 Maria Lizamba 泻湖、Arroyo Hondo 和 Amapa 河取样的水葫芦在 190 ℃下进行了 1、5 和 10 分钟的蒸汽爆炸预处理。处理 10 分钟后，从泻湖样品（5.4 克/50 克干物质）中获得了最高量的水葫芦的还原糖。蒸熟的生物质经固态发酵得到的酶水解，得到还原糖（最高15.5g/L）；酶解的效率为每克水葫芦 0.51 克还原糖。最后用还原糖发酵酿酒酵母转化为乙醇，乙醇浓度最高 (7.13 g/L)，乙醇产量为 0.23 g/g 干物质。