Modified nucleoside triphosphates in bacterial research for in vitro and live-cell applications,RSC Chemical Biology

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Modified nucleoside triphosphates in bacterial research for in vitro and live-cell applications
RSC Chemical Biology ( IF 4.2 ) Pub Date : 2020-09-14 , DOI: 10.1039/d0cb00078g
Adeline Espinasse ₁ , Hannah K Lembke ₁ , Angela A Cao ₁ , Erin E Carlson _{1,

2,

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Affiliation

Modified nucleoside triphosphates (NTPs) are invaluable tools to probe bacterial enzymatic mechanisms, develop novel genetic material, and engineer drugs and proteins with new functionalities. Although the impact of nucleobase alterations has predominantly been studied due to their importance for protein recognition, sugar and phosphate modifications have also been investigated. However, NTPs are cell impermeable due to their negatively charged phosphate tail, a major hurdle to achieving live bacterial studies. Herein, we review the recent advances made to investigate and evolve bacteria and their processes with the use of modified NTPs by exploring alterations in one of the three moieties: the nucleobase, the sugar and the phosphate tail. We also present the innovative methods that have been devised to internalize NTPs into bacteria for in vivo applications.

中文翻译：

修饰三磷酸核苷在体外和活细胞细菌研究中的应用

修饰的三磷酸核苷 (NTP) 是探索细菌酶机制、开发新型遗传物质以及设计具有新功能的药物和蛋白质的宝贵工具。尽管由于核碱基改变对蛋白质识别的重要性而主要研究了其影响，但糖和磷酸盐修饰也得到了研究。然而，NTP 由于其带负电荷的磷酸盐尾部而无法渗透细胞，这是实现活细菌研究的主要障碍。在此，我们通过探索核碱基、糖和磷酸尾这三个部分之一的改变，回顾了使用修饰的 NTP 来研究和进化细菌及其过程的最新进展。我们还介绍了将 NTP 内化到细菌中以进行体内应用的创新方法。

更新日期：2020-11-03

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