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Long non-coding RNA MIR4300HG polymorphisms are associated with postoperative nausea and vomiting: a genome-wide association study.
Human Genomics ( IF 3.8 ) Pub Date : 2020-09-14 , DOI: 10.1186/s40246-020-00282-4
Shigekazu Sugino 1, 2 , Daisuke Konno 1 , Yosuke Kawai 3 , Masao Nagasaki 3 , Yasuhiro Endo 1 , Tomo Hayase 2 , Misako Yamazaki-Higuchi 2 , Yukihiro Kumeta 2 , Shunsuke Tachibana 2 , Katsuhiko Saito 4 , Jun Suzuki 1 , Kanta Kido 5 , Nahoko Kurosawa 4 , Akiyoshi Namiki 6 , Masanori Yamauchi 1
Affiliation  

Genetic factors such as single-nucleotide polymorphisms (SNPs) play a key role in the development of postoperative nausea and vomiting (PONV). However, previous findings are not widely applicable to different populations because of population-specific genetic variation. We developed a Japanese-specific DNA microarray for high-throughput genotyping. The aim of the current study was to identify SNPs associated with PONV on a genome-wide scale using this microarray in a sample of Japanese surgical patients. Associations between 659,636 SNPs and the incidence of PONV 24 h after surgery in a limited sample of 24 female patients were assessed using the microarray. After imputation of genotypes at 24,330,529 SNPs, 78 SNPs were found to be associated with the incidence of PONV. We chose 4 of the 78 SNPs to focus on by in silico functional annotation. Finally, we genotyped these 4 candidate SNPs in 255 patients using real-time PCR to verify association with the incidence of PONV. The T > C variant of rs11232965 in the long non-coding RNA MIR4300HG was significantly associated with reduced incidence of PONV among genotypes and between alleles (p = 0.01 and 0.007). We identified a novel SNP (rs11232965) in the long non-coding RNA MIR4300HG that is associated with PONV. The rs11232965-SNP variant (T > C) is protective against the incidence of PONV. This study was registered at the UMIN Clinical Trials Registry (Identifier: UMIN000022903 , date of registration: June 27, 2016, retrospectively registered.

中文翻译:

长链非编码 RNA MIR4300HG 多态性与术后恶心和呕吐相关:一项全基因组关联研究。

单核苷酸多态性 (SNP) 等遗传因素在术后恶心和呕吐 (PONV) 的发展中起关键作用。然而,由于特定人群的遗传变异,以前的发现并不广泛适用于不同的人群。我们开发了一种用于高通量基因分型的日本特定 DNA 微阵列。当前研究的目的是在日本手术患者的样本中使用这种微阵列在全基因组范围内鉴定与 PONV 相关的 SNP。使用微阵列评估了 24 名女性患者的有限样本中 659,636 个 SNP 与手术后 24 小时 PONV 发生率之间的关联。在对 24,330,529 个 SNP 进行基因型插补后,发现 78 个 SNP 与 PONV 的发生率相关。我们通过计算机功能注释选择了 78 个 SNP 中的 4 个进行关注。最后,我们使用实时 PCR 对 255 名患者的这 4 个候选 SNP 进行基因分型,以验证与 PONV 发生率的关联。长链非编码 RNA MIR4300HG 中 rs11232965 的 T > C 变体与基因型和等位基因之间的 PONV 发生率降低显着相关(p = 0.01 和 0.007)。我们在与 PONV 相关的长链非编码 RNA MIR4300HG 中发现了一个新的 SNP (rs11232965)。rs11232965-SNP 变体 (T > C) 可防止 PONV 的发生。本研究在UMIN临床试验注册中心注册(编号:UMIN000022903,注册日期:2016年6月27日,追溯注册。长链非编码 RNA MIR4300HG 中 rs11232965 的 C 变体与基因型和等位基因之间的 PONV 发生率降低显着相关(p = 0.01 和 0.007)。我们在与 PONV 相关的长链非编码 RNA MIR4300HG 中发现了一个新的 SNP (rs11232965)。rs11232965-SNP 变体 (T > C) 可防止 PONV 的发生。本研究在UMIN临床试验注册中心注册(编号:UMIN000022903,注册日期:2016年6月27日,追溯注册。长链非编码 RNA MIR4300HG 中 rs11232965 的 C 变体与基因型和等位基因之间的 PONV 发生率降低显着相关(p = 0.01 和 0.007)。我们在与 PONV 相关的长链非编码 RNA MIR4300HG 中发现了一个新的 SNP (rs11232965)。rs11232965-SNP 变体 (T > C) 可防止 PONV 的发生。本研究在UMIN临床试验注册中心注册(编号:UMIN000022903,注册日期:2016年6月27日,追溯注册。
更新日期:2020-09-14
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