Proper regulation of homeotic gene expression is critical for stem cell fate in both plants and animals. In Arabidopsis thaliana, the WUSCHEL (WUS)‐RELATED HOMEOBOX 5 (WOX5) gene is specifically expressed in a group of root stem cell organizer cells called the quiescent center (QC) and plays a central role in QC specification. Here, we report that the SEUSS (SEU) protein, homologous to the animal LIM‐domain binding (LDB) proteins, assembles a functional transcriptional complex that regulates WOX5 expression and QC specification. SEU is physically recruited to the WOX5 promoter by the master transcription factor SCARECROW. Subsequently, SEU physically recruits the SET domain methyltransferase SDG4 to the WOX5 promoter, thus activating WOX5 expression. Thus, analogous to its animal counterparts, SEU acts as a multi‐adaptor protein that integrates the actions of genetic and epigenetic regulators into a concerted transcriptional program to control root stem cell organizer specification.

中文翻译：

---

SEUSS 整合了根干细胞组织者规范的转录和表观遗传控制。


同源异型基因表达的正确调节对于植物和动物干细胞的命运至关重要。在拟南芥中， WUSCHEL ( WUS )相关的同源盒 5 ( WOX5 ) 基因在一组称为静止中心 (QC) 的根干细胞组织细胞中特异性表达，并在 QC 规范中发挥核心作用。在这里，我们报道了与动物 LIM 结构域结合 (LDB) 蛋白同源的 SEUSS (SEU) 蛋白，组装了调节WOX5表达和 QC 规范的功能性转录复合物。 SEU 由主转录因子 SCARECROW 物理招募至WOX5启动子。随后，SEU 将 SET 结构域甲基转移酶 SDG4 物理招募至WOX5启动子，从而激活WOX5表达。因此，与动物对应物类似，SEU 充当多适配器蛋白，将遗传和表观遗传调节因子的作用整合到协调的转录程序中，以控制根干细胞组织者的规范。