Abstract

Seed oil traits in soybean that are of benefit to human nutrition and health have been selected for during crop domestication. However, these domesticated traits have significant differences across various evolutionary types. In this study, we found that the integration of evolutionary population structure (evolutionary types) with genome-wide association studies increased the power of gene detection, and it identified one locus for traits related to seed size and oil content on chromosome 13. This domestication locus, together with another one in a 200-kb region, was confirmed by the GEMMA and EMMAX software. The candidate gene, GmPDAT, had higher expressional levels in high-oil and large-seed accessions than in low-oil and small-seed accessions. Overexpression lines had increased seed size and oil content, whereas RNAi lines had decreased seed size and oil content. The molecular mechanism of GmPDAT was deduced based on results from linkage analysis for triacylglycerols and on histocytological comparisons of transgenic soybean seeds. Our results illustrate a new approach for identifying domestication genes with pleiotropic effects.

中文翻译：

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进化群体结构模型揭示了 GmPDAT 对大豆种子油和大小相关性状的多效性影响。

 抽象的

在作物驯化过程中选择了对人类营养和健康有益的大豆种子油性状。然而，这些驯化特征在不同的进化类型中具有显着差异。在这项研究中，我们发现进化种群结构（进化类型）与全基因组关联研究的整合提高了基因检测的能力，并在 13 号染色体上鉴定了一个与种子大小和含油量相关性状的基因座。 GEMMA 和 EMMAX 软件确认了该位点以及 200 kb 区域中的另一个位点。候选基因GmPDAT在高油和大种子种质中的表达水平高于低油和小种子种质。过表达品系增加了种子大小和含油量，而RNAi品系降低了种子大小和含油量。根据三酰基甘油的连锁分析结果和转基因大豆种子的组织细胞学比较，推导出GmPDAT的分子机制。我们的结果说明了一种识别具有多效性效应的驯化基因的新方法。