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DNA Origami Nanostructures with Scaffolds Obtained from Rolling Circle Amplification
ACS Materials Letters ( IF 9.6 ) Pub Date : 2020-09-14 , DOI: 10.1021/acsmaterialslett.9b00484
Mengyuan Xu 1 , Chi Zhang 1 , Cunjun Zhang 1 , Yiling Zhao 2 , Zhi Qi 2 , Chunhai Fan 3 , Jie Chao 4 , Bryan Wei 1
Affiliation  

As a guiding backbone of origami structural folding, the long single-stranded scaffold is the key enabler of all kinds of complex DNA nanostructures. However, the folding of most origami nanostructures depends heavily on very limited species of scaffolds. It is desirable to have a wider choice of scaffolds for the extended design space. Here we develop a new method based on rolling circle amplification to prepare scaffolds of custom lengths and sequences. We demonstrate that amplicons templated by viral genome and plasmid can serve as scaffolds to fold DNA origami nanostructures.

中文翻译:

DNA折纸纳米结构与支架的滚环扩增。

作为折纸结构折叠的指导性骨架,长单链支架是所有复杂DNA纳米结构的关键推动力。然而,大多数折纸纳米结构的折叠在很大程度上取决于非常有限的支架种类。对于扩展的设计空间,希望有更多的支架选择。在这里,我们开发了一种基于滚环扩增的新方法,以制备自定义长度和序列的支架。我们证明了由病毒基因组和质粒模板化的扩增子可以作为支架折叠DNA折纸纳米结构。
更新日期:2020-10-05
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