Abstract

The depletion of gut mucosal barrier enables exposure of gut microbes/gut microbial products to the host mucosal immunity which may increase the risk of metabolic/inflammatory disorders. These immune responses can lead to the development of mild autoimmunity to metabolic peptides coming from gut bacteria and may result in metabolic diseases like late-onset diabetes (LOD). In the present study, we identified host sera cross-reactivity with gut bacterial peptides similar to host proteins. The interaction between diabetic sera and gut peptides was detected by enzyme-linked immunosorbent assay (ELISA) and results were confirmed using surface plasmon resonance (SPR). The ELISA assay showed a higher level of serum cross-reactivity in LOD patients as compared to non-diabetic controls against three peptides (P-5, P-9, and P-13). SPR analysis confirmed binding-affinity against P-5 and P-13. Also, a significant correlation was observed between inflammatory markers and P-5. This study demonstrates that gut health is important not only for intestinal diseases but also for several late-onset diseases, like, diabetes.

摘要

肠道粘膜屏障的消耗使肠道微生物/肠道微生物产物暴露于宿主粘膜免疫，这可能会增加代谢/炎症性疾病的风险。这些免疫反应可导致对来自肠道细菌的代谢肽产生轻度自身免疫，并可能导致代谢疾病，如迟发性糖尿病 (LOD)。在本研究中，我们确定了宿主血清与类似于宿主蛋白质的肠道细菌肽的交叉反应性。通过酶联免疫吸附试验 (ELISA) 检测糖尿病血清和肠道肽之间的相互作用，并使用表面等离子体共振 (SPR) 确认结果。ELISA 测定显示，与非糖尿病对照相比，LOD 患者的血清交叉反应水平更高，针对三种肽（P-5、P-9 和 P-13）。SPR 分析证实了对 P-5 和 P-13 的结合亲和力。此外，在炎症标志物和 P-5 之间观察到显着相关性。这项研究表明，肠道健康不仅对肠道疾病很重要，而且对糖尿病等几种迟发性疾病也很重要。