Abstract

A new ent-kaurane-type diterpenoid, crotonmekongenin A (1) together with two known compounds, epifriedelanol (2) and sitosterol glucoside (3) from the leaves and twigs of Croton mekongensis were isolated. The structure of these compounds was determined by spectroscopic analysis. The structure of the new ent-kaurane-type diterpenoid (1) was elucidated using spectroscopic technique and X-ray crystallography analysis. Moreover, the cytotoxicity of all extract and isolated compounds was tested for cytotoxicity against KKU-M213, FaDu, HT-29, MDA-MB-231, A-549, SH-SY5Y, and CL cell lines. Compound 1 showed significant cytotoxic activity (ED₅₀) on FaDu (0.48 µg/ml), HT-29 (0.63 µg/ml), and SH-SY5Y (0.45 µg/ml).

摘要

从湄公河巴豆的叶子和枝条中分离到了一种新的ent-kaurane型二萜，巴豆木糖苷A ( 1 ) 以及两种已知的化合物表弗里德醇( 2 ) 和谷甾醇葡萄糖苷( 3 ) 。这些化合物的结构通过光谱分析确定。使用光谱技术和 X 射线晶体学分析阐明了新的 ent-kaurane 型二萜 ( 1 ) 的结构。此外，还测试了所有提取物和分离化合物对 KKU-M213、FaDu、HT-29、MDA-MB-231、A-549、SH-SY5Y 和 CL 细胞系的细胞毒性。化合物1显示出显着的细胞毒活性 (ED ₅₀) 在 FaDu (0.48 µg/ml)、HT-29 (0.63 µg/ml) 和 SH-SY5Y (0.45 µg/ml) 上。