Abstract

Scorzonera austriaca Wild is a traditional herbal medicine; however, little is known with regard to the effect of flavonoids from S. austriaca (FSA) on liver injury induced by Carbon tetrachloride (CCl₄), especially the mechanism remains unknown. Therefore, our paper was designed to investigate the hepatoprotective effect of FSA against CCl₄-induced acute liver injury in vitro and in vivo, with focus on its potential mechanism. The purity of FSA prepared by using polyporous resin column chromatography could reach 94.5%, and seven flavonoid compounds in FSA were identified by using LC-ESI-MS analysis. In vivo results showed that FSA markedly decreased the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and malonaldehyde (MDA) and increased the contents of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Furthermore, in vivo and in vitro results confirmed that FSA could inhibit inflammatory response, as evidenced by decreasing the levels of tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) through inactivating toll-like receptor-4/nuclear factor-κB (TLR4/NF-κB) signaling pathway. FSA activated autophagy by increasing the ratio of LC3B-II/I and decreasing the protein level of p62 so as to exert its hepatoprotective effect. In general, these evidences suggested that FSA is likely to serve as a potential material for the drugs against chemical hepatic injury.

摘要

Scorzonera austriaca Wild 是一种传统草药；然而，关于来自S. austriaca (FSA) 的黄酮类化合物对四氯化碳(CCl ₄ ) 引起的肝损伤的作用知之甚少，尤其是其机制尚不清楚。因此，本论文旨在研究FSA在体外和体内对CCl ₄诱导的急性肝损伤的保肝作用，重点研究其潜在机制。多孔树脂柱层析法制备的FSA纯度可达94.5%，LC-ESI-MS分析鉴定出FSA中的7种黄酮类化合物。体内结果表明，FSA显着降低了天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、乳酸脱氢酶（LDH）和丙二醛（MDA）的含量，增加了超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的含量。此外，在体内和体外结果证实 FSA 可以抑制炎症反应，这可以通过灭活 toll 样受体 4/核因子-κB (TLR4/ NF-κB) 信号通路。FSA通过增加LC3B-II/I比值和降低p62蛋白水平来激活自噬，从而发挥其保肝作用。总的来说，这些证据表明，FSA 很可能作为抗化学性肝损伤药物的潜在材料。