Alzheimer's disease (AD) accounts for an estimated 60% to 80% of all dementia cases. The present study is aimed at evaluating the neuroprotective efficacy of vitexin, an apigenin flavone glycoside using transgenic Caenorhabditis elegans strain (CL2006) of AD. The neuroprotective effect of vitexin was determined using physiological assays, quantitative polymerase chain reaction, and Western blotting. The results of survival and paralysis assay indicate that vitexin (200 μM) significantly extended the lifespan of the nematodes. Vitexin‐treated nematodes showed a significant reduction in the expression of Aβ, ace‐1, and ace‐2 genes when compared to control. Further, vitexin significantly upregulated the expression of acr‐8 and dnj‐14, and increased the lifespan of the nematodes. Vitexin was also found to modulate the unfolded protein response genes (hsp‐4, pek‐1, ire‐1, and xbp‐1) and suppress the expression of Aβ. Overall, the results show that vitexin acts as a neuroprotective agent and protects transgenic C. elegans strains from Aβ proteotoxicity.

中文翻译：

---

Vitexin通过调节未折叠的蛋白质反应，防止阿尔茨海默氏病转基因秀丽隐杆线虫模型中的Aβ蛋白毒性。

阿尔茨海默氏病（AD）估计占所有痴呆症病例的60％至80％。本研究旨在使用转基因秀丽隐杆线虫秀丽隐杆线虫菌株（CL2006）评估芹菜素黄酮苷vitexin的神经保护作用。使用生理学测定，定量聚合酶链反应和蛋白质印迹法确定了葡萄黄素的神经保护作用。存活和麻痹分析的结果表明，vitexin（200μM）显着延长了线虫的寿命。与对照相比，经线粒体处理的线虫显示出Aβ，ace-1和ace-2基因的表达显着降低。此外，vitexin显着上调了acr-8和dnj-14，并增加了线虫的寿命。还发现Vitexin调节未折叠的蛋白反应基因（hsp-4，pek-1，ire-1和xbp-1）并抑制Aβ的表达。总体而言，结果表明，葡萄黄素充当神经保护剂并保护转基因秀丽隐杆线虫菌株免受Aβ蛋白毒性。