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Corrigendum.
Genes to Cells ( IF 1.3 ) Pub Date : 2020-09-14 , DOI: 10.1111/gtc.12799


Ohnishi, T., Shirane, M., Hashimoto, Y., Saita, S., & Nakayama, K. (2014) Identification and characterization of a neuron‐specific isoform of protrudin. Genes to Cells. 19, 97–111.

In Experimental procedures, the first sentence under the sub‐heading “Construction of expression plasmids” should read as follows:

Construction of expression plasmids

Mouse cDNAs encoding protrudin‐S or protrudin‐L (Gen‐Bank Accession Numbers NM_001164531.1 and NM_177319.3, respectively) were generated by PCR with PrimeSTAR HS DNA polymerase (TaKaRa, Shiga, Japan) and specific primer pairs listed in Table 1 from cDNA preparations of the thymus and cerebrum, respectively, and they were subcloned into the p3 × FLAG‐CMV‐7.1 (Sigma, St Louis, MO, USA), pmCherry (Clontech, Palo Alto, CA, USA), or pcDNA3 (Invitrogen, Carlsbad, CA, USA) vectors.

Also, the email address of the corresponding author has changed.

The new email address should read as shiram@phar.nagoya-cu.ac.jp



中文翻译:

更正。

Ohnishi, T.、Shirane, M.、Hashimoto, Y.、Saita, S. 和 Nakayama, K.(2014)protrudin 的神经元特异性同种型的鉴定和表征。基因到细胞。 19 , 97–111。

在实验程序中,小标题“表达质粒的构建”下的第一句应如下所示:

表达质粒的构建

编码 protrudin-S 或 protrudin-L 的小鼠 cDNA(分别为 Gen-Bank 登录号 NM_001164531.1 和 NM_177319.3)通过 PCR 生成,使用 PrimeSTAR HS DNA 聚合酶(TaKaRa,Shiga,日本)和表 1 中列出的特定引物对分别来自胸腺和大脑的 cDNA 制剂,并将它们亚克隆到 p3 × FLAG-CMV-7.1 (Sigma, St Louis, MO, USA)、pmCherry (Clontech, Palo Alto, CA, USA) 或 pcDNA3 ( Invitrogen, Carlsbad, CA, USA) 载体。

此外,通讯作者的电子邮件地址已更改。

新的电子邮件地址应为 shiram@phar.nagoya-cu.ac.jp

更新日期:2020-09-14
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