Elasticity‐Driven Membrane Budding through Cholesterol Concentration on Supported Lipid Monolayer–Bilayer Junction,Advanced Materials Interfaces

当前位置： X-MOL 学术 › Adv. Mater. Interfaces › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Elasticity‐Driven Membrane Budding through Cholesterol Concentration on Supported Lipid Monolayer–Bilayer Junction
Advanced Materials Interfaces ( IF 4.3 ) Pub Date : 2020-09-13 , DOI: 10.1002/admi.202000937
Yeon Kyung Lee _{1,

2} , Eui‐Sang Yu ₁ , Dong June Ahn _{2,

3} , Yong‐Sang Ryu ₁

Affiliation

Membrane budding is an essential process during various biological activities, such as intercellular communication and transportation of life‐sustaining molecules and signals, which primarily occur with the aid of particular proteins. During such processes, the presence of cholesterol and its assembly into particular domains within the membranes have attracted considerable attention due to their unique characteristics in terms of the regulation of signal activities, membrane fluidity, and hormone production during metabolic pathways. However, despite these crucial roles, the precise mechanisms of their spatiotemporal localization toward specific areas and their physiological roles in membrane budding without the assistance of particular proteins remain unclear. Herein, a model membrane platform is reconstituted with lipid monolayer–bilayer junctions, which facilitate the selective concentration of cholesterol on the lipid monolayer regions. In vitro membrane budding is demonstrated by the remaining vesicles where the lipid monolayer membrane is ruptured. The physicochemical approach to the selective concentration of cholesterol and its consequent membrane vesiculation offer important clues to help understand the underlying mechanisms of cholesterol in the lipid‐driven membrane‐budding process.

中文翻译：

胆固醇在脂质单分子层-双层分子交界处的胆固醇驱动下的弹性驱动膜萌芽

膜出芽是各种生物活动（例如细胞间通讯以及维持生命的分子和信号的运输）过程中的必不可少的过程，这些过程主要是借助特定的蛋白质进行的。在这样的过程中，胆固醇的存在及其在膜内特定区域的组装由于其在信号途径，膜流动性和代谢途径中激素产生方面的独特调节而受到了广泛的关注。然而，尽管具有这些关键作用，但它们在特定区域的时空定位的精确机制及其在没有特定蛋白质帮助下在膜萌芽中的生理作用仍不清楚。在这里模型膜平台由脂质单层-双层连接重建，这有助于胆固醇在脂质单层区域上的选择性浓缩。脂质单层膜破裂的其余囊泡证明了体外膜出芽。采用物理化学方法对胆固醇进行选择性浓缩及其随后的膜囊泡化提供了重要的线索，以帮助了解胆固醇在脂质驱动的膜芽生过程中的潜在机制。

更新日期：2020-11-06

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11