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Combined Effects of Electric Stimulation and Microgrooves in Cardiac Tissue‐on‐a‐Chip for Drug Screening
Small Methods ( IF 10.7 ) Pub Date : 2020-09-13 , DOI: 10.1002/smtd.202000438 Li Ren 1 , Xingwu Zhou 1 , Rohollah Nasiri 1 , Jun Fang 1 , Xing Jiang 1 , Canran Wang 1 , Moyuan Qu 1 , Haonan Ling 1 , Yihang Chen 1 , Yumeng Xue 1 , Martin C Hartel 1 , Peyton Tebon 1 , Shiming Zhang 1 , Han-Jun Kim 1 , Xichen Yuan 1 , Amir Shamloo 2 , Mehmet Remzi Dokmeci 1 , Song Li 3 , Ali Khademhosseini 1 , Samad Ahadian 1 , Wujin Sun 1
Small Methods ( IF 10.7 ) Pub Date : 2020-09-13 , DOI: 10.1002/smtd.202000438 Li Ren 1 , Xingwu Zhou 1 , Rohollah Nasiri 1 , Jun Fang 1 , Xing Jiang 1 , Canran Wang 1 , Moyuan Qu 1 , Haonan Ling 1 , Yihang Chen 1 , Yumeng Xue 1 , Martin C Hartel 1 , Peyton Tebon 1 , Shiming Zhang 1 , Han-Jun Kim 1 , Xichen Yuan 1 , Amir Shamloo 2 , Mehmet Remzi Dokmeci 1 , Song Li 3 , Ali Khademhosseini 1 , Samad Ahadian 1 , Wujin Sun 1
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Animal models and traditional cell cultures are essential tools for drug development. However, these platforms can show striking discrepancies in efficacy and side effects when compared to human trials. These differences can lengthen the drug development process and even lead to drug withdrawal from the market. The establishment of preclinical drug screening platforms that have higher relevancy to physiological conditions is desirable to facilitate drug development. Here, a heart‐on‐a‐chip platform, incorporating microgrooves and electrical pulse stimulations to recapitulate the well‐aligned structure and synchronous beating of cardiomyocytes (CMs) for drug screening, is reported. Each chip is made with facile lithographic and laser‐cutting processes that can be easily scaled up to high‐throughput format. The maturation and phenotypic changes of CMs cultured on the heart‐on‐a‐chip is validated and it can be treated with various drugs to evaluate cardiotoxicity and cardioprotective efficacy. The heart‐on‐a‐chip can provide a high‐throughput drug screening platform in preclinical drug development.
中文翻译：
电刺激和心脏组织芯片上微纹的联合效应用于药物筛选
动物模型和传统细胞培养是药物开发的重要工具。然而，与人体试验相比，这些平台在功效和副作用方面可能表现出惊人的差异。这些差异可能会延长药物开发过程，甚至导致药物退出市场。建立与生理条件相关性较高的临床前药物筛选平台有利于药物开发。在此，报道了一种芯片上的心脏平台，该平台结合了微槽和电脉冲刺激来重现心肌细胞（CM）的排列良好的结构和同步跳动，以进行药物筛选。每个芯片均采用简便的光刻和激光切割工艺制成，可以轻松扩展到高通量格式。在芯片心脏上培养的 CM 的成熟和表型变化得到了验证，并且可以用各种药物进行治疗，以评估心脏毒性和心脏保护功效。芯片上的心脏可以为临床前药物开发提供高通量药物筛选平台。
更新日期：2020-10-07
中文翻译：
电刺激和心脏组织芯片上微纹的联合效应用于药物筛选
动物模型和传统细胞培养是药物开发的重要工具。然而，与人体试验相比，这些平台在功效和副作用方面可能表现出惊人的差异。这些差异可能会延长药物开发过程，甚至导致药物退出市场。建立与生理条件相关性较高的临床前药物筛选平台有利于药物开发。在此，报道了一种芯片上的心脏平台，该平台结合了微槽和电脉冲刺激来重现心肌细胞（CM）的排列良好的结构和同步跳动，以进行药物筛选。每个芯片均采用简便的光刻和激光切割工艺制成，可以轻松扩展到高通量格式。在芯片心脏上培养的 CM 的成熟和表型变化得到了验证，并且可以用各种药物进行治疗，以评估心脏毒性和心脏保护功效。芯片上的心脏可以为临床前药物开发提供高通量药物筛选平台。
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