This study was performed to evaluate reproductive performance after non‐surgical embryo transfer (Ns‐ET) of 10–15 porcine expanded blastocysts (ExBs) that had been vitrified and warmed (V/W) using the micro volume air cooling (MVAC) method. The effect of asynchrony between the donor and recipient estrous cycle was investigated. Ns‐ET was conducted in recipients whose estrous cycle was asynchronous to that of donors by a delay of 2, 1, or 0 days. In the 2‐day and 1‐day groups, the similar farrowing rates (27.3% and 25.0%) and survival rates to term (13.9% and 15.7%) were obtained after Ns‐ET of V/W ExBs. None of the recipients in 0‐day group farrowed. Artificial insemination (AI) prior to Ns‐ET was then evaluated. Ten–15 V/W ExBs were transferred non‐surgically to 12 recipients whose estrous cycles were asynchronous to that of donors by a 2‐day delay. All of the recipients produced piglets, and all (100.0%) delivered piglets were derived from the transferred V/W ExBs. The survival rate of V/W ExBs to term was 25.2%. These results demonstrate that Ns‐ET of V/W ExBs using MVAC can facilitate piglet production, even if 10–15 embryos are transferred. Moreover, piglets were obtained stably when AI was performed prior to Ns‐ET.

中文翻译：

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受体母猪的授精提高了非手术转移的玻璃化和加热猪扩张胚泡的存活期。

本研究旨在评估 10-15 个猪膨胀囊胚 (ExBs) 在非手术胚胎移植 (Ns-ET) 后的生殖性能，这些囊胚已使用微体积空气冷却 (MVAC) 方法进行玻璃化和加热 (V/W) . 研究了供体和受体动情周期之间不同步的影响。Ns-ET 在发情周期与捐赠者的发情周期延迟 2、1 或 0 天不同步的接受者中进行。在 2 天和 1 天组中，在 V/W ExBs 的 Ns-ET 后获得了相似的分娩率（27.3% 和 25.0%）和足月存活率（13.9% 和 15.7%）。0 天组的接受者均未分娩。然后评估 Ns-ET 之前的人工授精 (AI)。10–15 V/W ExBs 以非手术方式转移给 12 名受者，这些受者的发情周期与捐赠者的发情周期有 2 天的延迟。所有接受者都生产了仔猪，并且所有 (100.0%) 交付的仔猪都来自转移的 V/W ExB。V/W ExBs 的存活率为 25.2%。这些结果表明，即使转移了 10-15 个胚胎，使用 MVAC 的 V/W ExB 的 Ns-ET 也可以促进仔猪生产。此外，在 Ns-ET 之前进行 AI 时，可以稳定获得仔猪。