In arid or semi‐arid regions, deep‐sowing is an effective treasure to ensure seeds absorbing water from deep soil layer at present. However, the existing maize varieties have poor tolerance to deep‐sowing, which is attributed to that few genes are explored and utilised. In this study, 243 IBM Syn4 recombinant inbred lines (RIL) constructed with B73 and Mo17 as parents and 1,339 DNA markers evenly distributed in 10 chromosomes, were used for QTL analysis of deep‐sowing tolerance during seed germination. There were significant differences in germination‐related traits between the parental lines at 12.5 cm sowing depth. Among them, 7, 7, 5, 10 and 2 QTLs for emergence rate, seedling length, plumule length, mesocotyl length and coleoptile length were detected, respectively. These QTLs explained 2.75% to 10.49% of the phenotypic variance with LOD scores ranging from 2.50 to 8.27. In addition, 12 overlapping QTLs formed five QTL clusters on chromosomes 3, 5, 7 and 9. This study provides a basis for molecular marker‐assisted breeding and functional study in deep‐sowing germination of maize.

中文翻译：

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玉米IBM Syn4 RIL群体种子发芽期间的深播耐受性的QTL分析

在干旱或半干旱地区，目前深播是确保种子从深层土壤中吸收水分的有效宝藏。但是，现有的玉米品种对深播的耐受性差，这归因于很少开发和利用基因。在这项研究中，以B73和Mo17为亲本构建的243个IBM Syn4重组自交系（RIL）和均匀分布在10条染色体上的1,339个DNA标记用于种子发芽期间对深播耐受性的QTL分析。在12.5 cm播种深度的亲本之间，发芽相关性状之间存在显着差异。其中，分别检测了7、7、5、10和2个QTL，分别针对出苗率，幼苗长度，胚芽长度，中胚轴长度和胚芽鞘长度。这些QTL解释了2.75％至10。表型差异的49％，LOD得分在2.50至8.27之间。此外，12个重叠的QTL在3号，5号，7号和9号染色体上形成5个QTL簇。该研究为分子标记辅助育种和玉米深播发芽的功能研究提供了基础。