This study investigated the acute toxicity (LC_50‐24 hr) effects of the essential oil of Cymbopogon citratus for adult Argulus sp. and Dolops discoidalis, before and during oviposition. In vitro acute toxicity (LC_50‐24 hr) was tested using 20, 40, 60, 80, 100, 120, 140 and 160 μg/L of C. citratus essential oil, and two control groups (one with cultivation tank water and one with cultivation tank water + alcohol) were used. Specimens of Argulus sp. and D. discoidalis submitted to acute toxicity were evaluated using histological procedures. The major chemical compounds of C. citratus essential oil were geranial (47.5%), neral (35.6%) and myrcene (6.7%). The LC_50‐24 hr for Argulus sp. adults was 67.97 μg/L, while for D. discoidalis it was 59.55 µg/L. In the oviposition of both species of argulids, maximum mortality began with treatments of 140 μg/L, while the LC_50‐24 hr for Argulus sp. and D. discoidalis was 83.98 μg/L and 82.48 μg/L, respectively. In both argulid species exposed to C. citratus essential oil, morphological alterations were observed only in the eyes, and they occurred in the ommatidium and rhabdomeres and were dependent on the concentration of C. citratus essential oil and the parasite species.

中文翻译：

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致死浓度的柠檬柏（Poaceae）精油对Dolops discoidalis和Argulus sp。的影响。（甲壳纲：古耳科）。

这项研究调查了急性毒性（LC _50-24小时）的精油的作用柠檬草成人Argulus SP。和Dolops discoidalis，之前和产卵期间。使用_{20、40、60、80、100、120、140}和160μg/ L_柠檬柠条精油和两个对照组（一个带有培养罐水和一个_冰激凌）对体外急性毒性（LC _{50-24 hr}）进行了测试。一种使用培养槽水+酒精）。Argulus sp。的标本 使用组织学方法评估了D.和盘状D. discoidalis的急性毒性。柠檬梭菌的主要化学成分香精油是香叶醛（47.5％），神经醛（35.6％）和月桂烯（6.7％）。该LC _50-24小时的Argulus SP。成人为67.97μg/ L，而盘状线虫为59.55μg/ L。在argulids的两个物种的产卵，最大死亡开始的140微克/升的治疗，而LC _50-24小时为Argulus藻。和D. discoidalis为83.98微克/ L和82.48微克/升。在暴露于两者argulid物种C. citratus精油，仅观察到在眼底形态学改变，并且它们发生在小眼和感杆并且是依赖于浓度C. citratus精油和寄生虫种类。