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Sputum versus nasopharyngeal samples for the molecular diagnosis of respiratory viral infection in cystic fibrosis: A pilot study
Journal of Cystic Fibrosis ( IF 5.4 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.jcf.2020.09.003
Emilie Cardot-Martin 1 , Hélène Le Guillou-Guillemette 2 , Rozenn Le Berre 3 , Sophie Ramel 4 , Jean Le Bihan 4 , Dominique Grenet 5 , Eric Farfour 1 , Françoise Troussier 6 , Thierry Urban 7 , Lisa Billard 8 , Léa Pilorgé 9 , Adissa Minoui-Tran 9 , Christopher Payan 10 , Marie-Reine Munck 11 , Geneviève Héry-Arnaud 12 , Sophie Vallet 10
Affiliation  

Viruses are important agents in lung function deterioration in Cystic Fibrosis (CF). To date, no standard operating procedures (SOPs) have been established to determine which sampling method is the most effective for an optimal virological diagnosis of respiratory viral infections in CF. Here we investigated the performances of two sampling sites, sputum samples versus nasopharyngeal (NP) swabs, for thirty participants from three CF centres presenting an acute respiratory infection. Sputum and NP samples were simultaneously collected and multiplex PCR targeting 16 to 18 viruses were performed. Viruses were detected for 18/30 patients (60%). A high concordance between the sputum and NP samples was observed in 25 (83%) paired samples of which 13 tested positive and 12 tested negative. These results highlighted the relevance of sputum sampling for diagnostic of respiratory viruses in CF, which is less invasive and better accepted by CF patients than NP, and allows accurate bacterial detection.

中文翻译:

用于囊性纤维化呼吸道病毒感染分子诊断的痰液与鼻咽样本:初步研究

病毒是囊性纤维化 (CF) 患者肺功能恶化的重要因素。迄今为止,尚未建立标准操作程序 (SOP) 来确定哪种采样方法对于 CF 呼吸道病毒感染的最佳病毒学诊断最有效。在这里,我们调查了来自三个 CF 中心的 30 名出现急性呼吸道感染的参与者的两个采样点的表现,痰样本与鼻咽 (NP) 拭子。同时收集痰和 NP 样本,并进行针对 16 至 18 种病毒的多重 PCR。18/30 名患者 (60%) 检测到病毒。在 25 个 (83%) 配对样本中观察到痰和 NP 样本之间的高度一致性,其中 13 个检测为阳性,12 个检测为阴性。
更新日期:2020-09-01
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