Selective detection of Cys and GSH by using one fluorescent probe with two different excitation wavelengths is developed based on a naphthyl-coumarin aldehyde probe. Mechanistic studies indicate that the distinctively different fluorescent responses of the probe toward Cys and GSH at the two excitation wavelengths are due to two different reaction pathways that generate different products. The probe has been used to detect Cys and GSH in HeLa cells by using two emission channels.

中文翻译：

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使用一个荧光探针在两个激发波长下选择性检测Cys和GSH

基于萘香豆素醛探针，开发了一种使用具有两种不同激发波长的荧光探针对Cys和GSH进行选择性检测的方法。机理研究表明，在两个激发波长下，探针对Cys和GSH的荧光响应显着不同是由于产生不同产物的两个不同反应途径。该探针已通过两个发射通道用于检测HeLa细胞中的Cys和GSH。