The recent pandemic outbreak of COVID-19 caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), poses a threat to public health globally. Thus, developing a rapid, accurate, and easy-to-implement diagnostic system for SARS-CoV-2 is crucial for controlling infection sources and monitoring illness progression. Here, we reported an ultrasensitive electrochemical detection technology using calixarene functionalized graphene oxide for targeting RNA of SARS-CoV-2. Based on a supersandwich-type recognition strategy, the technology was confirmed to practicably detect the RNA of SARS-CoV-2 without nucleic acid amplification and reverse-transcription by using a portable electrochemical smartphone. The biosensor showed high specificity and selectivity during in silico analysis and actual testing. A total of 88 RNA extracts from 25 SARS-CoV-2-confirmed patients and eight recovery patients were detected using the biosensor. The detectable ratios (85.5 % and 46.2 %) were higher than those obtained using RT-qPCR (56.5 % and 7.7 %). The limit of detection (LOD) of the clinical specimen was 200 copies/mL, which is the lowest LOD among the published RNA measurement of SARS-CoV-2 to date. Additionally, only two copies (10 μL) of SARS-CoV-2 were required for per assay. Therefore, we developed an ultrasensitive, accurate, and convenient assay for SARS-CoV-2 detection, providing a potential method for point-of-care testing.

最近由新型严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 引起的 COVID-19 大流行，对全球公共卫生构成威胁。因此，开发一种快速、准确且易于实施的 SARS-CoV-2 诊断系统对于控制感染源和监测疾病进展至关重要。在这里，我们报道了一种使用杯芳烃官能化氧化石墨烯靶向 SARS-CoV-2 的 RNA 的超灵敏电化学检测技术。基于超三明治型识别策略，该技术被证实可通过便携式电化学智能手机在无需核酸扩增和逆转录的情况下切实检测SARS-CoV-2的RNA。生物传感器在计算机中显示出高特异性和选择性分析和实际测试。使用生物传感器共检测到来自 25 名 SARS-CoV-2 确诊患者和 8 名康复患者的 88 份 RNA 提取物。可检测的比率（85.5 % 和 46.2 %）高于使用 RT-qPCR 获得的比率（56.5 % 和 7.7 %）。临床样本的检测限 (LOD) 为 200 拷贝/mL，这是迄今为止已发表的 SARS-CoV-2 RNA 测量值中最低的 LOD。此外，每次检测只需要两个拷贝 (10 μL) 的 SARS-CoV-2。因此，我们开发了一种超灵敏、准确、方便的 SARS-CoV-2 检测方法，为即时检测提供了一种潜在的方法。