In this work, we have developed an antifouling carbon surface by hydrogenating it using n-butylsilane reduction. After incubating physically small electrodes with such an antifouling carbon surface for 30 min in a synthetic fouling solution containing 1.0 % (v/v) caproic acid (a lipid), 4 % (w/v) bovine serum albumin and 0.01 % (w/v) cytochrome c (both are proteins), and 0.002 % (w/v) human fibrinopeptide B (a peptide), a ∼35 % lower dopamine detection signal was measured. However, no further significant change in dopamine detection signal was observed at electrodes that were incubated in the same synthetic fouling solution for one week, compared to a total loss of detection signal at non-hydrogenated carbon electrodes. We have also demonstrated the unique characteristic of these hydrogenated carbon electrodes in detecting dopamine with minimal interference from as high as 500 μM ascorbic acid that is generally expected in extracellular fluid. Meanwhile, there was also no observable fouling effect at hydrogenated carbon electrodes by the dopamine oxidation product, dopamine-o-quinone (itself a well-known fouling reagent), in the presence of ≤1.0 μM dopamine, which is a 100-fold higher concentration than that in the central nervous system. These results support minimal fouling at n-butylsilane hydrogenated carbon electrodes during dopamine detection in vitro.

在这项工作中，我们开发了一种防污碳表面，方法是使用n丁基硅烷还原。在含有1.0％（v / v）己酸（脂质），4％（w / v）牛血清白蛋白和0.01％（w / v）的合成污垢溶液中将具有此类防污碳表面的物理小电极孵育30分钟后v）细胞色素c（均为蛋白质）和0.002％（w / v）人血纤蛋白肽B（一种肽），测得的多巴胺检测信号降低了约35％。然而，与在非氢化碳电极上检测信号的总损失相比，在同一合成污垢溶液中孵育了一周的电极没有观察到多巴胺检测信号的进一步显着变化。我们还证明了这些氢化碳电极在检测多巴胺方面的独特特性，而对细胞外液中通常期望的高达500μM抗坏血酸的干扰却最小。同时，多巴胺氧化产物多巴胺-在氢化碳电极上也没有明显的结垢作用。邻醌（本身是一种著名的结垢试剂），在多巴胺≤1.0μM的情况下，其浓度比中枢神经系统高100倍。这些结果支持在体外多巴胺检测过程中对正丁基硅烷氢化碳电极的污染最小。