In present study, fungi isolated from metal rich site were explored for metal tolerance capacity and potential of multifarious synthesis of metal nanoparticles (NPs). Total 9 different fungi were isolated from rhizospeheric soils and molecularly characterized. Penicillium janthinellum DJP06 showed maximum metal tolerance capacity and synthesized gold and silver NPs. Detailed characterization confirmed the synthesis of protein capped, crystalline natured, spherical shaped gold and silver NPs. Mechanistic studies suggested involvement of fungal secretome proteins in the NPs synthesis. To find out the active protein(s) responsible for multifarious synthesis of NPs, the dialyzed proteins were fractionated by chromatography technique. Individual protein fractions were separately checked for their ability to synthesize NPs. Results suggested involvement of multiple proteins in an interactive manner for NPs synthesis. Testing the ability of these proteins for multifarious synthesis of other commercially important NPs will be an exciting future possibility.

在当前的研究中，从金属富集部位分离出的真菌具有金属耐受能力和金属纳米粒子（NPs）多种合成的潜力。从根茎土壤中分离出总共9种不同的真菌并进行了分子鉴定。青霉DJP06显示出最大的金属耐受能力以及合成的金和银NP。详细的表征证实了蛋白质加帽，结晶性质的球形金和银纳米颗粒的合成。机理研究表明真菌分泌蛋白参与了NP的合成。为了找出负责NP的多种合成的活性蛋白，通过色谱技术将透析的蛋白分级分离。单独检查各个蛋白质级分的合成NP的能力。结果表明多种蛋白质以相互作用的方式参与NP的合成。测试这些蛋白质对其他商业上重要的NP进行多种​​合成的能力将是一个令人兴奋的未来可能性。