This study was conducted to investigate the effects of phytase (Phy), benzoic acid (BA) and their interactions on growth performance, nutrients digestibility and tibia mineralization in broiler chickens fed standard or nutrient^,s deficient diets. A total of 768 one-d-old male chicks were divided into 8 treatments for 42 d. The treatments were as follows:positive control (PC), PC diet with 0.5 g/kg of BA, PC diet with 500 FTU/kg of Phy, PC diet with 0.5 g/kg of BA and 500 FTU/kg of Phy, negative control diet (NC) with a nutrient deficiency (ND) equal to matrix value of 500 FTU/kg of Phy, NC diet with 0.5 g/kg of BA, NC diet with 500 FTU/kg of Phy, and NC diet with 0.5 g/kg of BA and 500 FTU/kg of Phy. Broiler chickens received NC diets had lower body weight gain (BWG), feed intake (FI) and higher feed conversion ratio (FCR) in the entire experiment (P ≤ 0.05). Dietary ND negatively influenced tibia and toe ash contents, serum, and tibia P contents as well as ileal P, Ca and CP digestibility at d 28 (P ≤ 0.05). FCR of d 1 to 11 and d 12 to 24 improved when Phy was added to NC rather than PC diets (P ≤ 0.05). Phy supplementation did not affect serum P and Ca content at d 28 (P> 0.05), but reduced serum alkaline phosphatase content in broiler chickens fed PC diets (P ≤ 0.05). Phy supplementation increased tibia and toe ash contents at d 28, especially in broiler chickens fed NC diets (P ≤ 0.05). Inclusion of BA did not influence (P> 0.05) tibia ash, tibia Ca and P contents at d 28, but increased toe ash content (P ≤ 0.05).The interaction between BA and the dietary nutrient level was significant for FCR of d 25–42 and d 1–42 (P ≤ 0.05). Broiler chickens fed PC diets with BA had better FCR compared with those fed PC diets without BA atd 25–42 (P ≤ 0.05). Adding Phy to the diets containing BA improved BWG and FI atd 1–11(P ≤ 0.05). In conclusion, Phy was effective in improving some growth performance traits, and bone mineralization especially when added to NC diets. However, BA had a positive effect on FCR of broiler chickens fed PC diets and had no impact on ileal nutrients digestibility, but improved toe ash content.

这项研究旨在研究植酸酶（Phy），苯甲酸（BA）及其相互作用对饲喂标准饲料或营养物的肉鸡生长性能，营养物消化率和胫骨矿化的影响^，饮食不足。将总共​​768只一岁大的雄性小鸡分成8天，共处理42天。治疗方法如下：阳性对照（PC），含0.5 g / kg BA的PC饮食，含500 FTU / kg Phy的PC饮食，含0.5 g / kg BA和500 FTU / kg Phy的PC饮食，阴性营养素缺乏（ND）等于基质值500 FTU / kg Phy的对照饮食（NC），0.5 g / kg BA的NC饮食，500 FTU / kg Phy的NC饮食以及0.5 g的NC饮食/ kg BA和500 FTU / kg Phy。肉鸡接收NC饮食有较低的体重增加（BWG），饲料摄取量（FI）和在整个实验（较高的饲料转化率（FCR）P  ≤0.05）。日粮ND对第28天的胫骨和脚趾灰分含量，血清和胫骨P含量以及回肠P，Ca和CP消化率产生负面影响（P ≤0.05）。FCR d 1至11和d 12至24时物理层加入到NC，而不是PC饮食（改进的P  ≤0.05）。PHY补充不影响血清P和在d 28（Ca含量P肉鸡> 0.05），但是减少的血清碱性磷酸酶内容馈送PC日粮（P  ≤0.05）。PHY补充在d 28增加胫骨和脚趾灰分含量，特别是在供给NC日粮肉鸡（P  ≤0.05）。在第28天时，BA的加入并没有影响（P > 0.05）胫骨灰分，胫骨Ca和P的含量，但增加了脚趾灰分的含量（  P≤0.05）.BA与饮食营养水平的相互作用对d 25的FCR显着–42和d 1–42（P ≤0.05）。与饲喂PC日粮不BA相比供给PC日粮BA肉鸡有更好的FCR ATD 25-42（P  ≤0.05）。添加物理层设备到含有BA饮食改善BWG和FI ATD 1-11（P  ≤0.05）。总之，Phy可有效改善某些生长性能特征和骨骼矿化作用，尤其是添加到NC饮食中时。然而，BA对饲喂PC日粮的肉鸡的FCR有积极作用，对回肠养分的消化率没有影响，但改善了脚趾灰分含量。