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Toxicological implications of amplifying the antibacterial activity of gallic acid by immobilisation on silica particles: A study on C. elegans.
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2020-09-14 , DOI: 10.1016/j.etap.2020.103492
Samuel Verdú 1 , María Ruiz-Rico 1 , Alberto J Perez 2 , José M Barat 1 , Pau Talens 1 , Raúl Grau 1
Affiliation  

Immobilisation of natural compounds on solid supports to amplify antimicrobial properties has reported successful results, but modifications to physico-chemical properties can also imply modifications from a toxicological viewpoint. This work aimed to study the immobilising process of gallic acid in the antibacterial activity of L. innocua and its toxicological properties in vivo using Caenorhabditis elegans. The experiment was based on obtaining the minimum bactericidal concentration for free and immobilised gallic acid by comparing lethality, locomotion behaviour, chemotaxis and thermal stress resistance on C.elegans at those concentrations. The results showed a lowering minimum bactericidal concentration and modifications to nematode responses. Increased lethality and velocity of movements was observed. Immobilisation increased the repellent effect of gallic acid with a negative chemotaxis index. Thermal stress resistance was also affected, with higher mortality for immobilised gallic acid compared to bare particles and free gallic acid. Thus despite evidencing a generalised increase in the toxicity of gallic acid in vivo, lowering the minimum bactericidal concentration allowed a bacterial reduction of 99 % with less than one third of mortality for the nematodes exposed to free gallic acid.



中文翻译:

通过固定在二氧化硅颗粒上来放大没食子酸的抗菌活性的毒理学意义:秀丽隐杆线虫的研究。

已经报道了将天然化合物固定在固体载体上以增强抗菌性能的成功结果,但是从毒理学的角度来看,对物理化学性能的修饰也可能意味着修饰。这项工作的目的是研究没食子酸的固定化过程中的抗菌活性无害利斯特氏菌和其毒理学性质体内使用秀丽隐杆线虫。该实验是通过比较线虫线虫的致死性,运动行为,趋化性和耐热性,获得游离和固定的没食子酸的最低杀菌浓度。在那些浓度。结果表明降低了最低杀菌浓度并修饰了线虫反应。观察到杀伤力和移动速度增加。固定化增加了没食子酸趋化指数的没食子酸的排斥作用。耐热应力也受到影响,固定的没食子酸与裸颗粒和游离没食子酸相比死亡率更高。因此,尽管证明了没食子酸在体内的毒性普遍增加,但降低最低杀菌剂浓度仍可使细菌减少99%,而暴露于游离没食子酸的线虫的死亡率不到三分之一。

更新日期:2020-09-20
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