There are promising data on the use of poly(acrylamide) nanoparticles (PAA-NPs) in the development of third generation photosensitizers for application in antimicrobial photodynamic therapy. These nanoparticle delivery systems are suitable for biological applications and have potential for drug delivery due to their biocompatibility, chemical flexibility, easy preparation and low cost.

The objective of this work was to obtain new PAA-NPs for the vehiculization of the monobrominated derivatives of Azure B (AzBBr) and Neutral Red (NRBr). These two dyes were found to have promising properties as photosensitizers. However, the use of nanotechnology was required to overcome difficulties in solubility, aggregation and stability in order to optimize their photodynamic efficiency.

The combination of different monomers and crosslinkers allowed us to develop two new PAA-NPs. These systems were loaded with AzBBr and NRBr and characterized according to their morphology, size, polydispersity index and zeta potential. The new third generation photosensitizers showed better photochemical reactivity, chemical stability and photodynamic efficacy against Gram-positive and Gram-negative bacteria than the free photosensitizers.

The use of this vehiculization strategy improved the properties of the second generation photosensitizers (AzBBr and NRBr) studied. This improvement in their properties makes them good candidates for potential phototherapeutic applications against numerous pathologies.

关于在开发用于抗菌光动力疗法的第三代光敏剂方面，使用聚丙烯酰胺纳米颗粒（PAA-NPs）的数据很有希望。这些纳米颗粒递送系统由于其生物相容性，化学柔韧性，易于制备和低成本而适合于生物应用并且具有药物递送的潜力。

这项工作的目的是获得新的PAA-NP，用于将天青B（AzBBr）和中性红（NRBr）的单溴化衍生物汽车化。发现这两种染料具有光敏剂的前景。但是，需要使用纳米技术来克服溶解度，聚集和稳定性方面的困难，以优化其光动力效率。

不同单体和交联剂的结合使我们能够开发出两种新的PAA-NP。这些系统装有AzBBr和NRBr，并根据其形态，大小，多分散指数和Zeta电位进行了表征。新型第三代光敏剂对革兰氏阳性和革兰氏阴性细菌的光化学反应性，化学稳定性和光动力功效均优于游离光敏剂。

这种车辆化策略的使用改善了所研究的第二代光敏剂（AzBBr和NRBr）的性能。其性能的提高使它们成为针对多种病理的潜在光疗应用的良好候选者。