High-frequency spinal cord stimulation (HFSCS) at 10 kHz provides paresthesia-free treatment for chronic pain. However, the underlying mechanisms of its action have not been fully elucidated. The aim of the present study was to investigate the effect of HFSCS treatment on spinal glutamate release and uptake in spared nerve injury (SNI) rats. HFSCS was applied to the T10/T11 spinal cord 3 days after SNI. The concentration of spinal glutamate, glutamate transporter activity and miniature excitatory postsynaptic currents (mEPSCs) from neurons in lamina II were evaluated. HFSCS treatment alleviated SNI pain induced by mechanical and cold allodynia. HFSCS treatment also partially restored altered spinal glutamate uptake activity, the levels of spinal glutamate, and the frequency of mEPSCs following SNI. In conclusion, HFSCS treatment attenuated SNI-induced neuropathic pain and partially restored the altered glutamate uptake after SNI.

10 kHz 的高频脊髓刺激 (HFSCS) 为慢性疼痛提供无感觉异常治疗。然而，其作用的潜在机制尚未完全阐明。本研究的目的是研究 HFSCS 治疗对神经损伤 (SNI) 大鼠脊髓谷氨酸释放和摄取的影响。SNI 后 3 天将 HFSCS 应用于 T10/T11 脊髓。评估脊髓谷氨酸浓度、谷氨酸转运蛋白活性和来自第二层神经元的微型兴奋性突触后电流 (mEPSCs)。HFSCS 治疗减轻了机械性和冷性异常性疼痛引起的 SNI 疼痛。HFSCS 治疗还部分恢复了改变的脊髓谷氨酸摄取活性、脊髓谷氨酸水平和 SNI 后 mEPSCs 的频率。综上所述，