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Synchronization of stage of follicle development before OPU improves embryo production in cows with large antral follicle counts.
Animal Reproduction Science ( IF 2.2 ) Pub Date : 2020-09-14 , DOI: 10.1016/j.anireprosci.2020.106601
Sheila Merlo Garcia 1 , Fábio Morotti 2 , Fábio Luiz Bim Cavalieri 3 , Paula Alvares Lunardelli 4 , Aline de Oliveira Santos 1 , Claudia Maria Bertan Membrive 5 , Caliê Castilho 1 , Raquel Zaneti Puelker 6 , José Otávio Folino Silva 7 , Amanda Fonseca Zangirolamo 2 , Marcelo Marcondes Seneda 2
Affiliation  

In the present study, there was an evaluation of in vitro embryo production (IVEP) in Bos indicus donor cows with small or large antral follicle counts (AFCs) when there was synchronization of follicular dynamics among cows before ovum pick-up (OPU). Donor cows classified as having small or large AFC were submitted to OPU/IVEP program (Experiment-I) or had follicular-stage synchronization imposed before OPU/IVEP (Experiment-II). In Experiment-I, the cows with a large AFC had a greater (P < 0.01) mean of embryos developing to the blastocyst stage compared to those with a small AFC. In Experiment-II, percentage of viable oocytes/OPU were not affected (P = 0.33) by synchronization of follicular dynamics, but the AFC had an effect (P < 0.0001). There was an interaction (P = 0.01) indicating the larger AFC, with or without imposing of a synchronization treatment regimen, resulted in the most desirable outcome. The number of embryos was affected (P < 0.001) by follicular-stage synchronization and AFC, with there being an interaction (P = 0.002) with the most desirable results for the large AFC-synchronized group. Number of pregnancies was greater (P ≤ 0.02) for recipient females with embryos from synchronized donors and with a large AFC. There was an interaction (P = 0.03) with there being a greater pregnancy percentage for cows with synchronized follicular stages and the large AFC. Bos indicus donor with a large AFC when associated with the synchronization of stage of follicular dynamics pre-OPU results in improvement of the efficacy of IVEP.



中文翻译:

OPU 前卵泡发育阶段的同步提高了具有大量窦卵泡计数的奶牛的胚胎产量。

在本研究中,当在取卵 (OPU) 前奶牛之间的卵泡动力学同步时,对具有小或大窦卵泡计数 (AFC) 的Bos indicus供体奶牛的体外胚胎生产 (IVEP)进行了评估。被归类为具有小型或大型 AFC 的供体奶牛被提交给 OPU/IVEP 程序(实验-I)或在 OPU/IVEP 之前进行卵泡阶段同步(实验-II)。在实验 I 中,与具有小 AFC 的奶牛相比,具有大 AFC 的奶牛的胚胎发育到囊胚阶段的平均值更高(P < 0.01)。在实验二中,卵泡动力学同步不影响活卵母细胞/OPU 的百分比(P = 0.33),但 AFC 有影响(P < 0.0001)。存在交互作用 ( P = 0.01) 表明较大的 AFC,无论是否采用同步治疗方案,都会产生最理想的结果。胚胎数量受到卵泡期同步和 AFC 的影响(P < 0.001),其中相互作用(P = 0.002)与大 AFC 同步组最理想的结果。具有来自同步供体的胚胎和大 AFC 的受者女性的怀孕次数更多 ( P ≤ 0.02)。有一种交互作用(P = 0.03),具有同步卵泡阶段和大 AFC 的奶牛的妊娠百分比更高。木薯 当与 OPU 前卵泡动力学阶段的同步相关时,具有大 AFC 的供体可提高 IVEP 的功效。

更新日期:2020-09-20
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