Abstract

A novel technological approach based on ion implantation for obtaining nanostructured substrates for the visual characterization of ultrasmall biological objects and microorganisms has been described. By means of argon-ion implantation of silicate glasses through surface masks made of copper wire mesh, periodic microstructures in the form of gratings with mesh sizes of 50 × 50 μm were formed. The novel substrates were tested by scanning electron microscopy and energy-dispersive X-ray analysis, as well as atomic force microscopy, using bacteria of the genera Bacillus and Staphylococcus deposited onto their surface.

中文翻译：

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用于计数由离子注入形成的细菌的微结构化基质

摘要

已经描述了一种基于离子注入的新颖技术方法，该技术方法用于获得纳米结构的基底，用于视觉表征超小型生物物体和微生物。通过通过由铜丝网制成的表面掩膜的氩离子注入硅酸盐玻璃，形成了网眼尺寸为50×50μm的呈光栅形式的周期性微结构。使用沉积在其表面上的芽孢杆菌属和葡萄球菌属细菌，通过扫描电子显微镜和能量色散X射线分析以及原子力显微镜对新型基质进行了测试。