Key message

This study is the first to demonstrate that GA₄-induced dormancy release is associated with the NF-Y complex, which interacts with gibberellin inhibitor RGL2 in Japanese apricot.

Abstract

Seasonal dormancy is not only vital for the survival in cold winter but also affects flowering of temperate fruit trees and the dormancy release depends on the accumulation of the cold temperatures (Chilling requirement-CR). To understand the mechanism of dormancy release in deciduous fruit crops, we compared miRNA sequencing data during the transition stage from paradormancy to dormancy release in the Japanese apricot and found that the miR169 family showed significant differentially up-regulated expression during dormancy induction and was down-regulated during the dormancy release periods. The 5′ RACE assay and RT-qPCR validated its target gene NUCLEAR FACTOR-Y subunit A (NF-YA), which exhibited the opposite expression pattern. Further study showed that exogenous GA₄ could inhibit the expression of the gibberellic acid (GA) signal transduction suppressor PmRGL2 (RGA-LIKE 2) and promote the expression of NF-Y. Moreover, the interaction between the NF-Y family and GA inhibitor PmRGL2 was verified by the yeast-two-hybrid (Y2H) system and a bimolecular fluorescence complementarity (BiFC) experiment. These results suggest that synergistic regulation of the NF-Y and PmRGL2 complex leads to the activation of dormancy release induced by GA₄. These findings will help to elucidate the functional and regulatory roles of miR169 and NF-Y complex in seasonal bud dormancy induced by GA in Japanese apricot and provide new insights for the discovery of dormancy release mechanisms in woody plants.

中文翻译：

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miR169 和 PmRGL2 协同调节 NF-Y 复合物以激活日本杏的休眠释放 (Prunus mume Sieb. et Zucc.)。

关键信息

本研究首次证明 GA ₄诱导的休眠释放与日本杏中的赤霉素抑制剂 RGL2 相互作用的 NF-Y 复合物有关。

抽象的

季节性休眠不仅对寒冷冬季的生存至关重要，而且影响温带果树的开花，而休眠的释放取决于寒冷温度的积累（Chilling requirements-CR）。为了了解落叶果类作物的休眠释放机制，我们比较了日本杏从休眠到休眠释放过渡阶段的 miRNA 测序数据，发现 miR169 家族在休眠诱导期间表现出显着差异上调的表达，并且在在休眠释放期间进行调节。5' RACE 测定和 RT-qPCR 验证了其靶基因NUCLEAR FACTOR-Y 亚基 A ( NF-YA )，其表现出相反的表达模式。进一步研究表明，外源性 GA ₄能抑制赤霉酸（GA）信号转导抑制因子PmRGL2（RGA-LIKE 2）的表达，促进NF-Y的表达。此外，通过酵母双杂交（Y2H）系统和双分子荧光互补性（BiFC）实验验证了NF-Y家族与GA抑制剂PmRGL2之间的相互作用。这些结果表明，NF-Y 和 PmRGL2 复合物的协同调节导致 GA _{4诱导的休眠释放的激活。}. 这些发现将有助于阐明miR169和NF-Y复合物在日本杏GA诱导的季节性芽休眠中的功能和调控作用，并为发现木本植物休眠释放机制提供新的见解。