Present study explores the potential of seismo-mechanical stress in alleviating the macro- and micro-morphological aberrations observed in the in vitro regenerated plantlets of Vitex negundo L. (Chaste tree). Untreated in vitro plantlets showed non-functional stomata, trichomes, and photosynthetic pigments. This study demonstrates that these structural and physiological anomalies are altered to near natural normalcy by application of a deliberate seismic stress (mechanical agitation) before ex vitro transplantation. The stress could be applied to the rooted in vitro plantlets by placing them on an incubator shaker set at 40, 60, 80 and 100 revolutions per minute (rpm) for 2–16 minutes twice a day during 4 weeks of experiment. The treated plantlets on evaluation revealed that a mechanical shaking of plantlets at 80 rpm for 12 min has induced optimal increase in shoot number (48.0 ± 0.32), leaves per shoot (12.8 ± 0.26) and fresh weight (4.8 g) as compared to the control (non-stressed). The treatment also improved the photosynthetic pigment ratio (Chl a, b and total carotenoids) thus, making them resemble normal plantlets (4.00 ± 0.30 µg g⁻¹ FW). The foliar micro-morphological analysis revealed that the seismic stress helps in the development of structurally organized functional stomata, epidermal cells and trichomes in the in vitro derived plants. This is the first report on the use of seismic stress for alleviating the in vitro induced abnormalities and acclimatization of V. negundo plantlets. The study paves the way for rapid production of healthy plants by tissue culture and their acclimatization in a short time period for conservation and sustainable utilization of medicinal plants.

目前的研究探讨了地震机械应力在减轻黄荆子体外再生小植株中观察到的宏观和微观形态畸变方面的潜力。L.（Chaste树）。未经处理的体外苗显示无功能的气孔，毛状体和光合色素。这项研究表明，通过在体外移植之前施加有意的地震应力（机械搅拌），可以将这些结构和生理异常更改为接近自然常态。在实验的4周中，每天两次将其置于设定为40、60、80和100转/分钟（rpm）的恒温振荡器上，可对生根的体外小苗施加压力，持续2-16分钟。经评估，处理过的小植株显示，与80 rpm相比，以80 rpm的机械摇动小植株诱导了枝条数（48.0±0.32），单枝叶片（12.8±0.26）和鲜重（4.8 g）的最佳增加。控制（无压力）。a，b和总类胡萝卜素），因此它们类似于正常的幼苗（4.00±0.30 µg g ^-1 FW）。叶的微观形态分析表明，地震应力有助于体外衍生植物中结构组织的功能性气孔，表皮细胞和毛状体的发育。这是关于使用地震应力减轻体外诱导的V. negundo幼苗异常和适应环境的第一份报告。该研究为通过组织培养及其在短时间内适应环境，以保护和可持续利用药用植物，为健康植物的快速生产铺平了道路。