Microtubers (MT) are an innovative approach for nuclear seed production in potato (Solanum tuberosum L.). They are produced under in vitro conditions using different protocols. However, content and type of growth media as well as explant types significantly affect the MT production efficiency. This study was conducted to develop an efficient protocol for MT production by evaluating different growth regulators at different concentrations, types of growth media and explants in two separate experiments. In Experiment 1, the effects of six different growth regulators, and their three application rates on MT formation were compared. The effects of two growth media and three explant types on MT production were compared in Experiment 2. As a result, our studies indicated that cotton based liquid MS medium containing 0.1 mg L⁻¹ Thidiazuron, and using whole plants as explant can be used for efficient MT production in potato.

微型薯（MT）是一种用于马铃薯（Solanum tuberosum）核种生产的创新方法L.）。它们是在体外条件下使用不同的方案生产的。但是，生长培养基的含量和类型以及外植体类型会显着影响MT的生产效率。通过在两个单独的实验中评估不同浓度，生长培养基类型和外植体的不同生长调节剂，进行这项研究以开发出有效的MT生产方案。在实验1中，比较了6种不同的生长调节剂及其3种施用量对MT形成的影响。在实验2中比较了两种生长培养基和三种外植体类型对MT产生的影响。结果，我们的研究表明，棉基液态MS培养基含有0.1 mg L ^-1 噻二唑隆以及将整个植物用作外植体可用于马铃薯的高效MT生产。