Photoacoustic (PA) imaging (or optoacoustic imaging) is a novel biomedical imaging method in biological and medical research. This modality performs morphological, functional, and molecular imaging with and without labels in both microscopic and deep tissue imaging domains. A variety of innovations have enhanced 3D PA imaging performance and thus has opened new opportunities in preclinical and clinical imaging. However, the 3D visualization tools for PA images remains a challenge. There are several commercially available software packages to visualize the generated 3D PA images. They are generally expensive, and their features are not optimized for 3D visualization of PA images. Here, we demonstrate a specialized 3D visualization software package, namely 3D Photoacoustic Visualization Studio (3D PHOVIS), specifically targeting photoacoustic data, image, and visualization processes. To support the research environment for visualization and fast processing, we incorporated 3D PHOVIS onto the MATLAB with graphical user interface and developed multi-core graphics processing unit modules for fast processing. The 3D PHOVIS includes following modules: (1) a mosaic volume generator, (2) a scan converter for optical scanning photoacoustic microscopy, (3) a skin profile estimator and depth encoder, (4) a multiplanar viewer with a navigation map, and (5) a volume renderer with a movie maker. This paper discusses the algorithms present in the software package and demonstrates their functions. In addition, the applicability of this software to ultrasound imaging and optical coherence tomography is also investigated. User manuals and application files for 3D PHOVIS are available for free on the website (www.boa-lab.com). Core functions of 3D PHOVIS are developed as a result of a summer class at POSTECH, “High-Performance Algorithm in CPU/GPU/DSP, and Computer Architecture.” We believe our 3D PHOVIS provides a unique tool to PA imaging researchers, expedites its growth, and attracts broad interests in a wide range of studies.

光声（PA）成像（或光声成像）是生物和医学研究中的一种新型生物医学成像方法。这种模式可以在微观和深层组织成像领域中进行带有或不带有标记的形态，功能和分子成像。各种创新技术增强了3D PA成像性能，从而为临床前和临床成像带来了新的机遇。但是，用于PA图像的3D可视化工具仍然是一个挑战。有几种市售软件包可以可视化生成的3D PA图像。它们通常很昂贵，并且其功能并未针对PA图像的3D可视化进行优化。在这里，我们演示了专门针对光声数据的专用3D可视化软件包，即3D光声可视化Studio（3D PHOVIS），图像和可视化过程。为了支持可视化和快速处理的研究环境，我们将3D PHOVIS结合到具有图形用户界面的MATLAB上，并开发了用于快速处理的多核图形处理单元模块。3D PHOVIS包括以下模块：（1）马赛克体生成器；（2）用于光学扫描光声显微镜的扫描转换器；（3）皮肤轮廓估计器和深度编码器；（4）具有导航图的多平面查看器；以及（5）具有电影制作者的体绘制器。本文讨论了软件包中存在的算法并演示了其功能。此外，还研究了该软件对超声成像和光学相干断层扫描的适用性。3D PHOVIS的用户手册和应用文件可在网站（www.boa-lab.com）上免费获得。3D PHOVIS的核心功能是在POSTECH的夏季课程中开发的，“ CPU / GPU / DSP中的高性能算法和计算机体系结构”。我们相信，我们的3D PHOVIS为PA影像研究人员提供了独特的工具，可加快其发展，并引起广泛研究的广泛兴趣。