Synthetic scaffolds exhibiting bone repair ability equal to that of autogenous bone are required in the fields of orthopedics and dentistry. A suitable synthetic bone graft substitute should induce osteogenic differentiation of mesenchymal stem cells, osteogenesis, and angiogenesis. In this study, three types of honeycomb blocks (HCBs), composed of hydroxyapatite (HAp), β-tricalcium phosphate (TCP), and carbonate apatite (CO₃Ap), were fabricated, and the effects of HCB composition on bone formation and maturation were investigated. The HC structure was selected to promote cell penetration and tissue ingrowth. HAp and β-TCP HCBs were fabricated by extrusion molding followed by sintering. The CO₃Ap HCBs were fabricated by extrusion molding followed by sintering and dissolution-precipitation reactions. These HCBs had similar macroporous structures: all harbored uniformly distributed macropores (∼160 ​μm) that were regularly arrayed and penetrated the blocks unidirectionally. Moreover, the volumes of macropores were nearly equal (∼0.15 ​cm³/g). The compressive strengths of CO₃Ap, HAp, and β-TCP HCBs were 22.8 ​± ​3.5, 34.2 ​± ​3.3, and 24.4 ​± ​2.4 ​MPa, respectively. Owing to the honeycomb-type macroporous structure, the compressive strengths of these HCBs were higher than those of commercial scaffolds with intricate three-dimensional or unidirectional macroporous structure. Notably, bone maturation was markedly faster in CO₃Ap HCB grafting than in β-TCP and HAp HCB grafting, and the mature bone area percentages for CO₃Ap HCBs at postsurgery weeks 4 and 12 were 14.3- and 4.3-fold higher and 7.5- and 1.4-fold higher than those for HAp and β-TCP HCBs, respectively. The differences in bone maturation and formation were probably caused by the disparity in concentrations of calcium ions surrounding the HCBs, which were dictated by the inherent material resorption behavior and mechanism; generally, CO₃Ap is resorbed only by osteoclastic resorption, HAp is not resorbed, and β-TCP is rapidly dissolved even in the absence of osteoclasts. Besides the composition, the microporous structure of HC struts, inevitably generated during the formation of HCBs of various compositions, may contribute to the differences in bone maturation and formation.

在骨科和牙科领域需要具有与自体骨相同的骨修复能力的合成支架。合适的合成骨移植替代物应诱导间充质干细胞的成骨分化，成骨和血管生成。在这项研究中，制造了三种类型的蜂窝块（HCB），分别由羟基磷灰石（HAp），β-磷酸三钙（TCP）和碳酸盐磷灰石（CO ₃ Ap）组成，并且HCB组成对骨形成和生长的影响。成熟度进行了调查。选择HC结构以促进细胞渗透和组织向内生长。HAp和β-TCPHCB通过挤压成型然后烧结制成。一氧化碳₃Ap HCB通过挤出成型，烧结和溶解沉淀反应来制造。这些六氯代苯具有类似的大孔结构：全部都包含均匀分布的大孔（〜160μm），这些孔规则排列并单向穿透块。此外，大孔的体积几乎相等（〜0.15 cm ³ / g）。CO ₃ Ap，HAp和β-TCPHCB的抗压强度分别为22.8±3.5、34.2±3.3和24.4±2.4 MPa。由于呈蜂窝状的大孔结构，这些六氯代苯的抗压强度高于具有复杂的三维或单向大孔结构的商业支架。值得注意的是，CO _3中的骨成熟明显更快Ap HCB嫁接比β-TCP和HAp HCB嫁接要高，并且术后3和4周CO ₃ Ap HCB的成熟骨面积百分比分别比HAp高14.3和4.3倍，7.5和1.4倍和β-TCPHCB。骨成熟和形成的差异可能是由于六氯代苯周围钙离子浓度的差异引起的，这是由内在的物质吸收行为和机理决定的。通常，CO ₃Ap仅通过破骨细胞吸收而被吸收，HAp不被吸收，并且即使在没有破骨细胞的情况下，β-TCP也被迅速溶解。除了组成之外，在各种组成的HCB形成过程中不可避免地会产生HC撑杆的微孔结构，这可能会导致骨骼成熟和形成方面的差异。